ArticleRNA (New York, N.Y.)2009
A critical assessment of the utility of protein-free splicing systems.
Article in RNA (New York, N.Y.), 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
9 citing papers in PubMed, 21 citations in OpenAlex.
- Lights, camera, action! Capturing the spliceosome and pre-mRNA splicing with single-molecule fluorescence microscopy.Wiley interdisciplinary reviews. RNA · 2016Review
- Locus-specific epigenetic remodeling controls addiction- and depression-related behaviors.Nature neuroscience · 2014Article
- RNA catalyses nuclear pre-mRNA splicing.Nature · 2013Article
- RNA structure analysis of human spliceosomes reveals a compact 3D arrangement of snRNAs at the catalytic core.The EMBO journal · 2013Article
- Spliceosome structure and function.Cold Spring Harbor perspectives in biology · 2011Review
- Model systems: how chemical biologists study RNA.Current opinion in chemical biology · 2009Review
- The spliceosome as ribozyme hypothesis takes a second step.Proceedings of the National Academy of Sciences of the United States of America · 2009Article
- Identification and characterization of a short 2'-3' bond-forming ribozyme.RNA (New York, N.Y.) · 2009Article
- The use of simple model systems to study spliceosomal catalysis.RNA (New York, N.Y.) · 2009Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
U2 and U6 snRNAs form part of the catalytic spliceosome and represent strong candidates for components of its active site. Over the past decade it has become clear that these snRNAs are capable of catalyzing several different chemical reactions, leading to the widespread conclusion that the spliceosome is a ribozyme. Here, we discuss the advances in both protein-free and fully spliceosomal systems that would be required to conclude that the reactions observed to be catalyzed by protein-free snRNAs are related to splicing and question the reliability of snRNA-only systems as tools for mechanistic splicing research.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.