ArticleRNA (New York, N.Y.)2009
The use of simple model systems to study spliceosomal catalysis.
Article in RNA (New York, N.Y.), 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
6 citing papers in PubMed.
- Lights, camera, action! Capturing the spliceosome and pre-mRNA splicing with single-molecule fluorescence microscopy.Wiley interdisciplinary reviews. RNA · 2016Review
- A role for H/ACA and C/D small nucleolar RNAs in viral replication.Molecular biotechnology · 2014Article
- RNA catalyses nuclear pre-mRNA splicing.Nature · 2013Article
- Model systems: how chemical biologists study RNA.Current opinion in chemical biology · 2009Review
- Physical and genetic interactions of yeast Cwc21p, an ortholog of human SRm300/SRRM2, suggest a role at the catalytic center of the spliceosome.RNA (New York, N.Y.) · 2009Article
- The spliceosome as ribozyme hypothesis takes a second step.Proceedings of the National Academy of Sciences of the United States of America · 2009Article
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2 authors.
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Abstract
Since direct analysis of many aspects of spliceosomal function is greatly hindered by the daunting complexity of the spliceosome, the development of functionally validated simple model systems can be of great value. The critical role played by a base-paired complex of U6 and U2 snRNAs in splicing in vivo suggests that this complex could be a suitable starting point for the development of such a simple model system. However, several criteria must be satisfied before such a snRNA-based in vitro system can be considered a valid model for the spliceosomal catalytic core, including similarities at the level of reaction chemistry and cationic and sequence requirements. Previous functional analyses of in vitro assembled base-paired complexes of human U2 and U6 snRNAs have been promising, providing insight into catalysis. Furthermore, they strongly suggest that with further optimization, these RNAs might indeed be able to recapitulate the function of the spliceosomal catalytic core, thus opening the door to several lines of study not previously possible.
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Registered trials
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