ArticleMolecular and cellular biology2009
rRNA suppressor of a eukaryotic translation initiation factor 5B/initiation factor 2 mutant reveals a binding site for translational GTPases on the small ribosomal subunit.
Article in Molecular and cellular biology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 22 citations in OpenAlex.
- ADAR3 modulates neuronal differentiation and regulates mRNA stability and translation.Nucleic acids research · 2024Article
- Histone deacetylase Hos2 regulates protein expression noise by potentially modulating the protein translation machinery.Nucleic acids research · 2024Article
- A Novel Inhibitor of Translation Initiation Factor eIF5B inJournal of microbiology and biotechnology · 2024Article
- Role of aIF5B in archaeal translation initiation.Nucleic acids research · 2022Article
- Structural basis for the transition from translation initiation to elongation by an 80S-eIF5B complex.Nature communications · 2020Article
- Long-range interdomain communications in eIF5B regulate GTP hydrolysis and translation initiation.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Review
- Structure of the mammalian 80S initiation complex with initiation factor 5B on HCV-IRES RNA.Nature structural & molecular biology · 2014Article
- Molecular architecture of a eukaryotic translational initiation complex.Science (New York, N.Y.) · 2013Article
- Interaction between 25S rRNA A loop and eukaryotic translation initiation factor 5B promotes subunit joining and ensures stringent AUG selection.Molecular and cellular biology · 2013Article
- Translation initiation without IF2-dependent GTP hydrolysis.Nucleic acids research · 2012Article
- Proofreading of pre-40S ribosome maturation by a translation initiation factor and 60S subunits.Nature structural & molecular biology · 2012Article
- The structure and function of the eukaryotic ribosome.Cold Spring Harbor perspectives in biology · 2012Review
- Initiation factor eIF2γ promotes eIF2-GTP-Met-tRNAi(Met) ternary complex binding to the 40S ribosome.Nature structural & molecular biology · 2011Article
- Kinesin molecular motor Eg5 functions during polypeptide synthesis.Molecular biology of the cell · 2011Article
- Structural integrity of {alpha}-helix H12 in translation initiation factor eIF5B is critical for 80S complex stability.RNA (New York, N.Y.) · 2011Article
Corrections and comments
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Authors and funding
6 authors at 4 institutions in 1 country.
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Abstract
The translational GTPases promote initiation, elongation, and termination of protein synthesis by interacting with the ribosome. Mutations that impair GTP hydrolysis by eukaryotic translation initiation factor 5B/initiation factor 2 (eIF5B/IF2) impair yeast cell growth due to failure to dissociate from the ribosome following subunit joining. A mutation in helix h5 of the 18S rRNA in the 40S ribosomal subunit and intragenic mutations in domain II of eIF5B suppress the toxic effects associated with expression of the eIF5B-H480I GTPase-deficient mutant in yeast by lowering the ribosome binding affinity of eIF5B. Hydroxyl radical mapping experiments reveal that the domain II suppressors interface with the body of the 40S subunit in the vicinity of helix h5. As the helix h5 mutation also impairs elongation factor function, the rRNA and eIF5B suppressor mutations provide in vivo evidence supporting a functionally important docking of domain II of the translational GTPases on the body of the small ribosomal subunit.
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