ArticleInvestigative ophthalmology & visual science2009
Periplakin interactions with lens intermediate and beaded filaments.
Article in Investigative ophthalmology & visual science, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 18 citations in OpenAlex.
- Article
- Changes in DNA methylation hallmark alterations in chromatin accessibility and gene expression for eye lens differentiation.Epigenetics & chromatin · 2022Article
- CP49 and filensin intermediate filaments are essential for formation of cold cataract.Molecular vision · 2020Article
- SUMOylation of periplakin is critical for efficient reorganization of keratin filament network.Molecular biology of the cell · 2019Article
- Intermediate Filaments and the Plasma Membrane.Cold Spring Harbor perspectives in biology · 2017Review
- Spatially Directed Proteomics of the Human Lens Outer Cortex Reveals an Intermediate Filament Switch Associated With the Remodeling Zone.Investigative ophthalmology & visual science · 2016Article
- Differences in the distribution, phenotype and gene expression of subretinal microglia/macrophages in C57BL/6N (Crb1 rd8/rd8) versus C57BL6/J (Crb1 wt/wt) mice.Journal of neuroinflammation · 2015Article
- An alternative means of retaining ocular structure and improving immunoreactivity for light microscopy studies.Molecular vision · 2015Article
- Localization of complement factor H gene expression and protein distribution in the mouse outer retina.Molecular vision · 2015Article
- Rapid light-induced activation of retinal microglia in mice lacking Arrestin-1.Vision research · 2014Article
- Cholestasis induces reversible accumulation of periplakin in mouse liver.BMC gastroenterology · 2013Article
- Periaxin is required for hexagonal geometry and membrane organization of mature lens fibers.Developmental biology · 2011Article
- Functions of the intermediate filament cytoskeleton in the eye lens.The Journal of clinical investigation · 2009Review
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
purposeThe lens assembles two systems of intermediated filaments-vimentin intermediate filament (IF) and highly divergent, lens-specific beaded filament (BF)-sequentially as epithelial cells differentiate into fiber cells. The goal of this study was to identify linker proteins that integrate the different lens IF into the biology of the lens fiber cells.
methodsAntibodies to periplakin were used in coimmunoprecipitation studies to identify proteins that complex with BF and IF in detergent extracts of mouse lens. GST-periplakin fusion proteins were used to confirm coimmunoprecipitation
resultsYeast two-hybrid analysis was used to establish direct linkage between periplakin and BF/IF proteins and to narrow down binding domains. Immunocytochemistry was used to establish spatial and temporal coexpression of periplakin and BF/IF. results. Periplakin is found complexed to BF and IF in the lens. The COOH terminus of periplakin was shown to have a strong affinity for the CP49 rod 2 domain but not its head or rod 1 domains. Low-level affinity was seen between the filensin rod domain and periplakin. Periplakin localization in lens overlapped with BF and IF.
conclusionsDespite divergence in primary sequence, predicted secondary structure, and filament structure, CP49 has conserved the capacity to bind a common IF linker protein, periplakin, and shares that binding capacity with the other major lens IF protein, vimentin. This suggests that mutations in periplakin have the potential to emulate the cataract seen in lenses with defective BF proteins.
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