ArticleEukaryotic cell2009
Sphingolipid C-9 methyltransferases are important for growth and virulence but not for sensitivity to antifungal plant defensins in Fusarium graminearum.
Article in Eukaryotic cell, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed, 58 citations in OpenAlex.
- Two structurally different oomycete lipophilic microbe-associated molecular patterns induce distinctive plant immune responses.Plant physiology · 2024Article
- The biological functions of sphingolipids in plant pathogenic fungi.PLoS pathogens · 2023Review
- Genetic Characterization of the Acidic and Neutral Glycosphingolipid Biosynthetic Pathways inMicroorganisms · 2023Article
- Comparative transcriptomics identifies the keyFrontiers in genetics · 2023Article
- Phenotype to genotype inCurrent research in microbial sciences · 2022Article
- Glycosphingolipids in Filamentous Fungi: Biological Roles and Potential Applications in Cosmetics and Health Foods.Frontiers in microbiology · 2021Review
- Functions of Sphingolipids in Pathogenesis During Host-Pathogen Interactions.Frontiers in microbiology · 2021Review
- Biosynthesis of long chain base in sphingolipids in animals, plants and fungi.Future science OA · 2019Review
- Sphingolipid biosynthetic pathway is crucial for growth, biofilm formation and membrane integrity ofFuture medicinal chemistry · 2019Article
- Article
- Ionic Liquids Chemical Stress Triggers Sphingoid Base Accumulation inFrontiers in microbiology · 2019Article
- Identification and Characterization of Two New S-Adenosylmethionine-Dependent Methyltransferase Encoding Genes Suggested Their Involvement in Stipe Elongation ofMycobiology · 2019Article
- Membrane Sphingolipids Regulate the Fitness and Antifungal Protein Susceptibility ofFrontiers in microbiology · 2019Article
- Monohexosylceramides fromMolecules (Basel, Switzerland) · 2018Article
- Review
- The AGC Kinase YpkA Regulates Sphingolipids Biosynthesis and Physically Interacts With SakA MAP Kinase inFrontiers in microbiology · 2018Article
- Functional characterization of the Aspergillus nidulans glucosylceramide pathway reveals that LCB Δ8-desaturation and C9-methylation are relevant to filamentous growth, lipid raft localization and Psd1 defensin activity.Molecular microbiology · 2016Article
- Sphingolipids as targets for treatment of fungal infections.Future medicinal chemistry · 2016Review
- Transmembrane transporter expression regulated by the glucosylceramide pathway in Cryptococcus neoformans.BMC research notes · 2015Article
- Δ10(E)-Sphingolipid Desaturase Involved in Fusaruside Mycosynthesis and Stress Adaptation in Fusarium graminearum.Scientific reports · 2015Article
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The C-9-methylated glucosylceramides (GlcCers) are sphingolipids unique to fungi. They play important roles in fungal growth and pathogenesis, and they act as receptors for some antifungal plant defensins. We have identified two genes, FgMT1 and FgMT2, that each encode a putative sphingolipid C-9 methyltransferase (C-9-MT) in the fungal pathogen Fusarium graminearum and complement a Pichia pastoris C-9-MT-null mutant. The DeltaFgmt1 mutant produced C-9-methylated GlcCer like the wild-type strain, PH-1, whereas the DeltaFgmt2 mutant produced 65 to 75% nonmethylated and 25 to 35% methylated GlcCer. No DeltaFgmt1DeltaFgmt2 double-knockout mutant producing only nonmethylated GlcCer could be recovered, suggesting that perhaps C-9-MTs are essential in this pathogen. This is in contrast to the nonessential nature of this enzyme in the unicellular fungus P. pastoris. The DeltaFgmt2 mutant exhibited severe growth defects and produced abnormal conidia, while the DeltaFgmt1 mutant grew like the wild-type strain, PH-1, under the conditions tested. The DeltaFgmt2 mutant also exhibited drastically reduced disease symptoms in wheat and much-delayed disease symptoms in Arabidopsis thaliana. Surprisingly, the DeltaFgmt2 mutant was less virulent on different host plants tested than the previously characterized DeltaFggcs1 mutant, which lacks GlcCer synthase activity and produces no GlcCer at all. Moreover, the DeltaFgmt1 and DeltaFgmt2 mutants, as well as the P. pastoris strain in which the C-9-MT gene was deleted, retained sensitivity to the antifungal plant defensins MsDef1 and RsAFP2, indicating that the C-9 methyl group is not a critical structural feature of the GlcCer receptor required for the antifungal action of plant defensins.
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