ArticleReproduction (Cambridge, England)2009
Oocyte-specific deletion of complex and hybrid N-glycans leads to defects in preovulatory follicle and cumulus mass development.
Article in Reproduction (Cambridge, England), 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers, 1 of them a synthesis that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
17 citing papers in PubMed, 1 synthesis or guideline pooled it, 35 citations in OpenAlex.
- Pooled it
- Investigating autoimmunity in the etiology of premature ovarian insufficiency in a mouse model.Journal of reproductive immunology · 2026Article
- Oocyte-specific acylglycerol kinase loss leads to infertility in mice via mitochondrial dysfunction.Communications biology · 2026Article
- The Plasma Glycome Differences Between Women with PCOS and Healthy Controls.International journal of molecular sciences · 2026Article
- Phosphomannomutase 2-congenital disorder of glycosylation: exploring the role of N-glycosylation on the endocrine axes.Frontiers in endocrinology · 2025Review
- The neonatal southern white rhinoceros ovary contains oogonia in germ cell nests.Communications biology · 2023Article
- Rapid ovarian transcript changes during the onset of premature ovarian insufficiency.Reproduction & fertility · 2022Article
- Oocyte-specific disruption of adrenomedullin 2 gene enhances ovarian follicle growth after superovulation.Frontiers in endocrinology · 2022Article
- Tissue-specific Fixation Methods Are Required for Optimal In Situ Visualization of Hyaluronan in the Ovary, Kidney, and Liver.The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society · 2020Article
- Roles for Golgi Glycans in Oogenesis and Spermatogenesis.Frontiers in cell and developmental biology · 2019Review
- Molecular regulation of follicle-stimulating hormone synthesis, secretion and action.Journal of molecular endocrinology · 2018Review
- Dysregulation of follicle development in a mouse model of premature ovarian insufficiency.Reproduction (Cambridge, England) · 2016Article
- Association of gene polymorphisms in ABO blood group chromosomal regions and menstrual disorders.Experimental and therapeutic medicine · 2015Article
- Article
- Embryos generated from oocytes lacking complex N- and O-glycans have compromised development and implantation.Reproduction (Cambridge, England) · 2012Article
- Synthesis and secretion of gonadotropins including structure-function correlates.Reviews in endocrine & metabolic disorders · 2011Review
- Premature ovarian failure in mice with oocytes lacking core 1-derived O-glycans and complex N-glycans.Endocrinology · 2011Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Complex and hybrid N-glycans generated by N-acetylglucosaminyltransferase I (GlcNAcT-I), encoded by Mgat1, affect the functions of glycoproteins. We have previously shown that females with oocyte-specific deletion of a floxed Mgat1 gene using a zona pellucida protein 3 (ZP3)Cre transgene produce fewer pups primarily due to a reduction in ovulation rate. Here, we show that the ovulation rate of mutant females is decreased due to aberrant development of preovulatory follicles. After a superovulatory regime of 48 h pregnant mare's serum (PMSG) and 9 h human chorionic gonadotropin (hCG), mutant ovaries weighed less and contained approximately 60% fewer preovulatory follicles and more atretic and abnormal follicles than controls. Unlike controls, a proportion of mutant follicles underwent premature luteinization. In addition, mutant preovulatory oocytes exhibited gross abnormalities with approximately 36% being blebbed or zona-free. While 97% of wild-type oocytes had a perivitelline space at the preovulatory stage, approximately 54% of mutant oocytes did not. The cumulus mass surrounding mutant oocytes was also smaller with a decreased number of proliferating cells compared with controls, although hyaluronan around mutant oocytes was similar to controls. In addition, cumulus cells surrounding mutant eggs were resistant to removal by either hyaluronidase or incubation with capacitated sperm. Therefore, the absence of complex and hybrid N-glycans on oocyte glycoproteins leads to abnormal folliculogenesis resulting in a decreased ovulation rate.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.