ArticleJournal of bacteriology2009
Glucose- and glucokinase-controlled mal gene expression in Escherichia coli.
Article in Journal of bacteriology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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16 citing papers in PubMed, 25 citations in OpenAlex.
- A GTP-driven central carbon metabolism in the cellulolytic bacterium Ruminiclostridium cellulolyticum.Communications biology · 2025Article
- Characterizing lysine acetylation of glucokinase.Protein science : a publication of the Protein Society · 2024Article
- Characterization of a cold-adapted debranching enzyme and its role in glycogen metabolism and virulence of Vibrio vulnificus MO6-24/O.Journal of microbiology (Seoul, Korea) · 2022Article
- A comprehensive review on microbial production of 1,2-propanediol: micro-organisms, metabolic pathways, and metabolic engineering.Biotechnology for biofuels · 2021Review
- Secretome Analysis ofProteomes · 2021Article
- High-throughput mapping of the phage resistance landscape in E. coli.PLoS biology · 2020Article
- The interplay between effector binding and allostery in an engineered protein switch.Protein science : a publication of the Protein Society · 2016Article
- Transcriptome Analysis of Escherichia coli during dGTP Starvation.Journal of bacteriology · 2016Article
- Structural Basis for the Interconversion of Maltodextrins by MalQ, the Amylomaltase of Escherichia coli.The Journal of biological chemistry · 2015Article
- The α-glucan phosphorylase MalP of Corynebacterium glutamicum is subject to transcriptional regulation and competitive inhibition by ADP-glucose.Journal of bacteriology · 2015Article
- Genome-scale metabolic network reconstruction and in silico flux analysis of the thermophilic bacterium Thermus thermophilus HB27.Microbial cell factories · 2014Article
- Carbon catabolite repression correlates with the maintenance of near invariant molecular crowding in proliferating E. coli cells.BMC systems biology · 2013Article
- A bacterial glucanotransferase can replace the complex maltose metabolism required for starch to sucrose conversion in leaves at night.The Journal of biological chemistry · 2013Article
- Expression of the Lactobacillus plantarum malE gene is regulated by CcpA and a MalR-like protein.Journal of microbiology (Seoul, Korea) · 2011Article
- Role of maltose enzymes in glycogen synthesis by Escherichia coli.Journal of bacteriology · 2011Article
- Exposure to Glycolytic Carbon Sources Reveals a Novel Layer of Regulation for the MalT Regulon.International journal of microbiology · 2011Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
MalT is the central transcriptional activator of all mal genes in Escherichia coli. Its activity is controlled by the inducer maltotriose. It can be inhibited by the interaction with certain proteins, and its expression can be controlled. We report here a novel aspect of mal gene regulation: the effect of cytoplasmic glucose and glucokinase (Glk) on the activity and the expression of MalT. Amylomaltase (MalQ) is essential for the metabolism of maltose. It forms maltodextrins and glucose from maltose or maltodextrins. We found that glucose above a concentration of 0.1 mM blocked the activity of the enzyme. malQ mutants when grown in the absence of maltodextrins are endogenously induced by maltotriose that is derived from the degradation of glycogen. Therefore, the fact that glk malQ(+) mutants showed elevated mal gene expression finds its explanation in the reduced ability to remove glucose from MalQ-catalyzed maltodextrin formation and is caused by a metabolically induced MalQ(-) phenotype. However, even in mutants lacking glycogen, Glk controls endogenous induction. We found that overexpressed Glk due to its structural similarity with Mlc, the repressor of malT, binds to the glucose transporter (PtsG), releasing Mlc and thus increasing malT repression. In addition, even in mutants lacking Mlc (and glycogen), the overexpression of glk leads to a reduction in mal gene expression. We interpret this repression by a direct interaction of Glk with MalT concomitant with MalT inhibition. This repression was dependent on the presence of either maltodextrin phosphorylase or amylomaltase and led to the inactivation of MalT.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.