ArticleJournal of bacteriology2009
Influence of operator site geometry on transcriptional control by the YefM-YoeB toxin-antitoxin complex.
Article in Journal of bacteriology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 34 citations in OpenAlex.
- A haloarchaeal VapBC toxin-antitoxin system: regulatory mechanism and role in establishing population heterogeneity to facilitate survival of subpopulations under stress conditions.Applied and environmental microbiology · 2026Article
- VapC36-mediated intrinsic stress adaption is required for copper tolerance and pathogenesis ofScience advances · 2026Article
- Interactions of theMicroorganisms · 2021Article
- Insights into Transcriptional Repression of the Homologous Toxin-Antitoxin CassettesInternational journal of molecular sciences · 2020Article
- Stress Can Induce Transcription of Toxin-Antitoxin Systems without Activating Toxin.Molecular cell · 2020Article
- Regulation of Type II Toxin-Antitoxin Systems: The Translation-Responsive Model.Frontiers in microbiology · 2020Article
- Functional annotation of a novel toxin-antitoxin system Xn-RelT of Xenorhabdus nematophila; a combined in silico and in vitro approach.Journal of molecular modeling · 2017Article
- What Is the Link between Stringent Response, Endoribonuclease Encoding Type II Toxin-Antitoxin Systems and Persistence?Frontiers in microbiology · 2016Article
- Keeping the Wolves at Bay: Antitoxins of Prokaryotic Type II Toxin-Antitoxin Systems.Frontiers in molecular biosciences · 2016Review
- Expression of the Streptococcus pneumoniae yoeB chromosomal toxin gene causes cell death in the model plant Arabidopsis thaliana.BMC biotechnology · 2015Article
- Homology modeling and assigned functional annotation of an uncharacterized antitoxin protein from Streptomyces xinghaiensis.Bioinformation · 2015Article
- Escherichia coli antitoxin MazE as transcription factor: insights into MazE-DNA binding.Nucleic acids research · 2015Article
- Regulating toxin-antitoxin expression: controlled detonation of intracellular molecular timebombs.Toxins · 2014Review
- The axe-txe complex of Enterococcus faecium presents a multilayered mode of toxin-antitoxin gene expression regulation.PloS one · 2013Article
- Toxin-antitoxin loci vapBC-1 and vapXD contribute to survival and virulence in nontypeable Haemophilus influenzae.BMC microbiology · 2012Article
- Molecular structure and function of the novel BrnT/BrnA toxin-antitoxin system of Brucella abortus.The Journal of biological chemistry · 2012Article
- Crystal structure of the DNA-bound VapBC2 antitoxin/toxin pair from Rickettsia felis.Nucleic acids research · 2012Article
- Article
- Regulation of the vapBC-1 toxin-antitoxin locus in nontypeable Haemophilus influenzae.PloS one · 2012Article
- Recent advancements in toxin and antitoxin systems involved in bacterial programmed cell death.International journal of microbiology · 2010Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
YefM-YoeB is among the most prevalent and well-characterized toxin-antitoxin complexes. YoeB toxin is an endoribonuclease whose activity is inhibited by YefM antitoxin. The regions 5' of yefM-yoeB in diverse bacteria possess conserved sequence motifs that mediate transcriptional autorepression. The yefM-yoeB operator site arrangement is exemplified in Escherichia coli: a pair of palindromes with core hexamer motifs and a center-to-center distance of 12 bp overlap the yefM-yoeB promoter. YefM is an autorepressor that initially recognizes a long palindrome containing the core hexamer, followed by binding to a short repeat. YoeB corepressor greatly enhances the YefM-operator interaction. Scanning mutagenesis demonstrated that the short repeat is crucial for correct interaction of YefM-YoeB with the operator site in vivo and in vitro. Moreover, altering the relative positions of the two palindromes on the DNA helix abrogated YefM-YoeB cooperative interactions with the repeats: complex binding to the long repeat was maintained but was perturbed to the short repeat. Although YefM lacks a canonical DNA binding motif, dual conserved arginine residues embedded in a basic patch of the protein are crucial for operator recognition. Deciphering the molecular basis of toxin-antitoxin transcriptional control will provide key insights into toxin-antitoxin activation and function.
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