ArticleJournal of bacteriology2009
Lagging-strand DNA replication origins are required for conjugal transfer of the promiscuous plasmid pMV158.
Article in Journal of bacteriology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
21 citing papers in PubMed, 40 citations in OpenAlex.
- Dynamic evolution of ceftazidime-avibactam resistance due to interchanges betweenFrontiers in cellular and infection microbiology · 2023Article
- NDM-5-ProducingAnimals : an open access journal from MDPI · 2022Article
- Recognition of Streptococcal Promoters by the Pneumococcal SigA Protein.Frontiers in molecular biosciences · 2021Article
- Development of Strong Anaerobic Fluorescent Reporters for Clostridium acetobutylicum and Clostridium ljungdahlii Using HaloTag and SNAP-tag Proteins.Applied and environmental microbiology · 2020Article
- Combining Modules for Versatile and Optimal Labeling of Lactic Acid Bacteria: Two pMV158-Family Promiscuous Replicons, a Pneumococcal System for Constitutive or Inducible Gene Expression, and Two Fluorescent Proteins.Frontiers in microbiology · 2019Article
- A Dual-Replicon Shuttle Vector System for Heterologous Gene Expression in a Broad Range of Gram-Positive and Gram-Negative Bacteria.Current microbiology · 2018Article
- Colocation of the Multiresistance GeneAntimicrobial agents and chemotherapy · 2017Article
- Crosstalk between vertical and horizontal gene transfer: plasmid replication control by a conjugative relaxase.Nucleic acids research · 2017Article
- Discovery of a new family of relaxases in Firmicutes bacteria.PLoS genetics · 2017Article
- Replication of Staphylococcal Resistance Plasmids.Frontiers in microbiology · 2017Review
- Streptococcal group B integrative and mobilizable element IMESag-rpsI encodes a functional relaxase involved in its transfer.Open biology · 2016Article
- Identification of a Single Strand Origin of Replication in the Integrative and Conjugative Element ICEBs1 of Bacillus subtilis.PLoS genetics · 2015Article
- Integrative and Conjugative Elements (ICEs): What They Do and How They Work.Annual review of genetics · 2015Review
- Identification of a virulence-related surface protein XF in piscine Streptococcus agalactiae by pre-absorbed immunoproteomics.BMC veterinary research · 2014Article
- Mobilizable Rolling-Circle Replicating Plasmids from Gram-Positive Bacteria: A Low-Cost Conjugative Transfer.Microbiology spectrum · 2014Article
- Bringing them together: plasmid pMV158 rolling circle replication and conjugation under an evolutionary perspective.Plasmid · 2014Review
- Functional properties and structural requirements of the plasmid pMV158-encoded MobM relaxase domain.Journal of bacteriology · 2013Article
- Novel plasmid and its variant harboring both a bla(NDM-1) gene and type IV secretion system in clinical isolates of Acinetobacter lwoffii.Antimicrobial agents and chemotherapy · 2012Article
- Autoregulation of the synthesis of the MobM relaxase encoded by the promiscuous plasmid pMV158.Journal of bacteriology · 2012Article
- The MobM relaxase domain of plasmid pMV158: thermal stability and activity upon Mn2+ and specific DNA binding.Nucleic acids research · 2011Article
Corrections and comments
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Authors and funding
2 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The promiscuous streptococcal plasmid pMV158 is mobilizable by auxiliary plasmids and replicates by the rolling-circle mechanism in a variety of bacterial hosts. The plasmid has two lagging-strand origins, ssoA and ssoU, involved in the conversion of single-stranded DNA intermediates into double-stranded plasmid DNA during vegetative replication. Transfer of the plasmid also would involve conversion of single-stranded DNA molecules into double-stranded plasmid forms in the recipient cells by conjugative replication. To test whether lagging-strand origins played a role in horizontal transfer, pMV158 derivatives defective in one or in both sso's were constructed and tested for their ability to colonize new hosts by means of intra- and interspecies mobilization. Whereas either sso supported transfer between strains of Streptococcus pneumoniae, only plasmids that had an intact ssoU could be efficiently mobilized from S. pneumoniae to Enterococcus faecalis. Thus, it appears that ssoU is a critical factor for pMV158 promiscuity and that the presence of a functional sso plays an essential role in plasmid transfer.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.