ArticleJournal of virological methods2009
Human airway epithelial cell culture to identify new respiratory viruses: coronavirus NL63 as a model.
Article in Journal of virological methods, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
36 citing papers in PubMed, 47 citations in OpenAlex.
- Review
- Molecular mechanisms of human coronavirus NL63 infection and replication.Virus research · 2023Review
- Human Air-Liquid-Interface Organotypic Airway Cultures Express Significantly More ACE2 Receptor Protein and Are More Susceptible to HCoV-NL63 Infection than Monolayer Cultures of Primary Respiratory Epithelial Cells.Microbiology spectrum · 2022Article
- Cell and Animal Models for SARS-CoV-2 Research.Viruses · 2022Review
- Human airway lineages derived from pluripotent stem cells reveal the epithelial responses to SARS-CoV-2 infection.American journal of physiology. Lung cellular and molecular physiology · 2022Article
- High-throughput human primary cell-based airway model for evaluating influenza, coronavirus, or other respiratory viruses in vitro.Scientific reports · 2021Article
- Can cilia provide an entry gateway for SARS-CoV-2 to human ciliated cells?Physiological genomics · 2021Review
- In Vitro Lung Models and Their Application to Study SARS-CoV-2 Pathogenesis and Disease.Viruses · 2021Review
- Article
- Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2): a Systemic Infection.Clinical microbiology reviews · 2021Review
- In Vitro and In Vivo Models for Studying SARS-CoV-2, the Etiological Agent Responsible for COVID-19 Pandemic.Viruses · 2021Review
- Invited review: human air-liquid-interface organotypic airway tissue models derived from primary tracheobronchial epithelial cells-overview and perspectives.In vitro cellular & developmental biology. Animal · 2021Review
- Article
- Human Bocavirus 1 Infection of Well-Differentiated Human Airway Epithelium.Current protocols in microbiology · 2020Article
- Morphogenesis and cytopathic effect of SARS-CoV-2 infection in human airway epithelial cells.Nature communications · 2020Article
- Trypsin Treatment Unlocks Barrier for Zoonotic Bat Coronavirus Infection.Journal of virology · 2020Article
- The Lung, the Heart, the Novel Coronavirus, and the Renin-Angiotensin System; The Need for Clinical Trials.Frontiers in medicine · 2020Review
- Broad-spectrum coronavirus antiviral drug discovery.Expert opinion on drug discovery · 2019Review
- Air-Liquid Interface Method To Study Epstein-Barr Virus Pathogenesis in Nasopharyngeal Epithelial Cells.mSphere · 2018Article
- Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 4 institutions in 1 country.
Funding
Abstract
Propagation of new human respiratory virus pathogens in established cell lines is hampered by a lack of predictability regarding cell line permissivity and by availability of suitable antibody reagents to detect infection in cell lines that do not exhibit significant cytopathic effect. Recently, molecular methods have been used to amplify and identify novel nucleic acid sequences directly from clinical samples, but these methods may be hampered by the quantity of virus present in respiratory secretions at different time points following the onset of infection. Human airway epithelial (HAE) cultures, which effectively mimic the human bronchial environment, allow for cultivation of a wide variety of human respiratory viral pathogens. The goal of the experiments described here was to determine if propagation and identification of a human respiratory virus may be achieved through inoculation of HAE cultures followed by whole transcriptome amplification (WTA) and sequence analysis. To establish proof-of-principle human coronavirus NL63 (HCoV-NL63) was evaluated, and the first visualization of HCoV-NL63 virus by transmission electron microscopy (TEM) is reported. Initial propagation of human respiratory secretions onto HAE cultures followed by TEM and WTA of culture supernatant may be a useful approach for visualization and detection of new human respiratory pathogens that have eluded identification by traditional approaches.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.