ArticleMolecular and biochemical parasitology2009
Selection of yeast strains with enhanced expression of Plasmodium falciparum proteins.
Article in Molecular and biochemical parasitology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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8 citing papers in PubMed, 18 citations in OpenAlex.
- Biosynthesis of heme O in intraerythrocytic stages of Plasmodium falciparum and potential inhibitors of this pathway.Scientific reports · 2019Article
- Heavy path mining of protein-protein associations in the malaria parasite.Methods (San Diego, Calif.) · 2015Article
- Predicting and exploring network components involved in pathogenesis in the malaria parasite via novel subnetwork alignments.BMC systems biology · 2015Article
- A library of functional recombinant cell-surface and secreted P. falciparum merozoite proteins.Molecular & cellular proteomics : MCP · 2013Article
- A novel subnetwork alignment approach predicts new components of the cell cycle regulatory apparatus in Plasmodium falciparum.BMC bioinformatics · 2013Article
- Comparative Genomics and Systems Biology of Malaria ParasitesCurrent bioinformatics · 2012Article
- Module-based subnetwork alignments reveal novel transcriptional regulators in malaria parasite Plasmodium falciparum.BMC systems biology · 2012Article
- A densely overlapping gene fragmentation approach improves yeast two-hybrid screens for Plasmodium falciparum proteins.Molecular and biochemical parasitologyArticle
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Authors and funding
3 authors at 3 institutions in 1 country.
Funding
Abstract
The poor expression of Plasmodium falciparum proteins in heterologous systems and the difficulty in obtaining sufficient material directly from the parasite have limited the experimental characterization of many of the approximately 5200 proteins encoded by this organism. To improve the expression of P. falciparum proteins in the yeast Saccharomyces cerevisiae, we selected yeast ura3 mutants that acquired the ability to utilize the P. falciparum orthologue (PfOMPDC) of URA3 to grow on media lacking uracil. Two of these mutant strains, BY#29 and PJ#17, expressed up to 100-fold more of four P. falciparum proteins as a result of mutations in either HRP1 or KAP104, respectively. These mutations, as well as a temperature-sensitive rna15 mutation, likely decrease the efficiency of mRNA 3' end formation and produce longer mRNAs of P. falciparum genes. These yeast strains may be useful for the analysis and purification of P. falciparum proteins.
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