ReviewChemphyschem : a European journal of chemical physics and physical chemistry2009
DCDHF fluorophores for single-molecule imaging in cells.
Review in Chemphyschem : a European journal of chemical physics and physical chemistry, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
What it found
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
24 citing papers in PubMed.
- Exploring the excited state mechanisms of a viscosity-sensitive zeromethine merocyanine dye emitter.Chemical science · 2026Article
- A Fluorogenic Reaction for Monitoring Cross-Coupling with Turn-On Ratio Greater than a Hundred Thousand.Chemical & biomedical imaging · 2026Article
- Branching Ionizable Lipids Can Enhance the Stability, Fusogenicity, and Functional Delivery of mRNA.Small science · 2023Article
- Chemical and Biological Tools for the Study of Voltage-Gated Sodium Channels in Electrogenesis and Nociception.Chembiochem : a European journal of chemical biology · 2022Review
- Single-molecule fluorescence detection of a tricyclic nucleoside analogue.Chemical science · 2020Article
- Tunable Non-linear Refraction Properties and Ultrafast Excited State Dynamics of Dicyanomethylene Dihydrofuran Derivative.Frontiers in chemistry · 2020Article
- Switchable Fluorophores for Single-Molecule Localization Microscopy.Chemical reviews · 2018Review
- An Environmentally Sensitive Fluorescent Dye as a Multidimensional Probe of Amyloid Formation.The journal of physical chemistry. B · 2016Article
- Characterization of novel perylene diimides containing aromatic amino acid side chains.Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy · 2016Article
- Fluorescence microscopy visualization of contacts between objects.Angewandte Chemie (International ed. in English) · 2015Article
- Article
- Single molecule fluorescence approaches shed light on intracellular RNAs.Chemical reviews · 2014Review
- Small molecules aimed at type III secretion systems to inhibit bacterial virulence.MedChemComm · 2013Article
- Fluorescent saxitoxins for live cell imaging of single voltage-gated sodium ion channels beyond the optical diffraction limit.Chemistry & biology · 2012Article
- Fluorescent penetration enhancers for transdermal applications.Journal of controlled release : official journal of the Controlled Release Society · 2012Article
- Azido push-pull fluorogens photoactivate to produce bright fluorescent labels.The journal of physical chemistry. B · 2010Article
- Superresolution imaging of targeted proteins in fixed and living cells using photoactivatable organic fluorophores.Journal of the American Chemical Society · 2010Article
- Single-molecule spectroscopy and imaging of biomolecules in living cells.Analytical chemistry · 2010Article
- Monitoring protein interactions and dynamics with solvatochromic fluorophores.Trends in biotechnology · 2010Review
- Synchronously amplified fluorescence image recovery (SAFIRe).The journal of physical chemistry. B · 2010Article
Corrections and comments
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Authors and funding
15 authors.
Funding
Abstract
There is a persistent need for small-molecule fluorescent labels optimized for single-molecule imaging in the cellular environment. Application of these labels comes with a set of strict requirements: strong absorption, efficient and stable emission, water solubility and membrane permeability, low background emission, and red-shifted absorption to avoid cell autofluorescence. We have designed and characterized several fluorophores, termed "DCDHF" fluorophores, for use in live-cell imaging based on the push-pull design: an amine donor group and a 2-dicyanomethylene-3-cyano-2,5-dihydrofuran (DCDHF) acceptor group, separated by a pi-rich conjugated network. In general, the DCDHF fluorophores are comparatively photostable, sensitive to local environment, and their chemistries and photophysics are tunable to optimize absorption wavelength, membrane affinity, and solubility. Especially valuable are fluorophores with sophisticated photophysics for applications requiring additional facets of control, such as photoactivation. For example, we have reengineered a red-emitting DCDHF fluorophore so that it is dark until photoactivated with a short burst of low-intensity violet light. This molecule and its relatives provide a new class of bright photoactivatable small-molecule fluorophores, which are needed for super-resolution imaging schemes that require active control (here turning-on) of single-molecule emission.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.