ArticleMicrocirculation (New York, N.Y. : 1994)2009
Event-tracking model of adhesion identifies load-bearing bonds in rolling leukocytes.
Article in Microcirculation (New York, N.Y. : 1994), 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 14 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
14 citing papers in PubMed.
- EndothelialArteriosclerosis, thrombosis, and vascular biology · 2022Article
- Rolling neutrophils form tethers and slings under physiologic conditions in vivo.Journal of leukocyte biology · 2018Article
- Microfluidics-based side view flow chamber reveals tether-to-sling transition in rolling neutrophils.Scientific reports · 2016Article
- Applications of computational models to better understand microvascular remodelling: a focus on biomechanical integration across scales.Interface focus · 2015Review
- Neutrophil rolling at high shear: flattening, catch bond behavior, tethers and slings.Molecular immunology · 2013Review
- Multiscale computational models of complex biological systems.Annual review of biomedical engineering · 2013Review
- 'Slings' enable neutrophil rolling at high shear.Nature · 2012Article
- Leukocyte rolling on P-selectin: a three-dimensional numerical study of the effect of cytoplasmic viscosity.Biophysical journal · 2012Article
- Live cell imaging of paxillin in rolling neutrophils by dual-color quantitative dynamic footprinting.Microcirculation (New York, N.Y. : 1994) · 2011Article
- Biomechanics of leukocyte rolling.Biorheology · 2011Review
- Quantitative dynamic footprinting microscopy reveals mechanisms of neutrophil rolling.Nature methods · 2010Article
- Nano-motion dynamics are determined by surface-tethered selectin mechanokinetics and bond formation.PLoS computational biology · 2009Article
- Action at a distance: lengthening adhesion bonds with poly(ethylene glycol) spacers enhances mechanically stressed affinity for improved vascular targeting of microparticles.Langmuir : the ACS journal of surfaces and colloids · 2009Article
- Dynamics of Microvillus Extension and Tether Formation in Rolling Leukocytes.Cellular and molecular bioengineering · 2009Article
Corrections and comments
- Erratum issued
Authors and funding
4 authors.
Funding
Abstract
objectivesP-selectin binding to P-selectin glycoprotein ligand-1 (PSGL)-1 mediates leukocyte rolling under conditions of inflammation and injury. The aims of this study were to develop an efficient, high temporal resolution model for direct simulation of leukocyte rolling and conduct a study of load-bearing bonds using the model. MATERIALS AND
methodsA stochastic pi-calculus-driven event-tracking model of adhesion (ETMA) was developed and compared with experimental data. Multiple simulations for each case were conducted to obtain high-confidence numerical characteristics of leukocyte rolling.
resultsLeukocyte rolling and the underlying P-selectin-PSGL-1 bonds were studied under low wall shear rate (25-50 s(-1)) conditions from measured parameters of leukocyte rolling and bond properties. For the first time, the location, number, lifetime, history, and kinetics of load-bearing bonds and their influence on cell rolling were identified and instantaneous cell displacements, translational and rotational velocities, and cell-substrate distances derived. The model explains the commonly observed "stop-start" type rolling behavior and reveals that a few load-bearing bonds are sufficient to support rolling, while a large number of bonds dissociate before becoming load bearing.
conclusionsETMA provides a method for more precise, direct simulation of leukocyte rolling at low wall shear rates and sets a foundation upon which further refinements can be introduced.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.