ArticleLab on a chip2008
Neutrophil migration assay from a drop of blood.
Article in Lab on a chip, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
34 citing papers in PubMed, 65 citations in OpenAlex.
- Exclusive liquid repellency isolation (ELRi) microliter scale isolation technique unmasks T-cell migration defects to CCL21 in pediatric asthma.bioRxiv : the preprint server for biology · 2026Article
- Effects of Pressure, Hypoxia, and Hyperoxia on Neutrophil Granulocytes.Biomolecules · 2024Article
- Microliter Whole Blood Neutrophil Assay Preserving Physiological Lifespan and Functional Heterogeneity.Small methods · 2024Article
- Micro blood analysis technology (μBAT): multiplexed analysis of neutrophil phenotype and function from microliter whole blood samples.Lab on a chip · 2024Article
- [Inertial label-free sorting and chemotaxis of polymorphonuclear neutrophil in sepsis patients based on microfluidic technology].Sheng wu yi xue gong cheng xue za zhi = Journal of biomedical engineering = Shengwu yixue gongchengxue zazhi · 2023Article
- Passive redirection filters minimize red blood cell contamination during neutrophil chemotaxis assays using whole blood.Lab on a chip · 2023Article
- Simplified Cell Magnetic Isolation Assisted SCMicromachines · 2022Article
- Tissue Models forFrontiers in cellular and infection microbiology · 2022Review
- Prospects and Opportunities for Microsystems and Microfluidic Devices in the Field of Otorhinolaryngology.Clinical and experimental otorhinolaryngology · 2021Review
- Microfluidic devices for neutrophil chemotaxis studies.Journal of translational medicine · 2020Review
- Light-inducible activation of cell cycle progression in Xenopus egg extracts under microfluidic confinement.Lab on a chip · 2019Article
- MBiosensors & bioelectronics · 2018Article
- Neutrophil Chemotaxis in One Droplet of Blood Using Microfluidic Assays.Methods in molecular biology (Clifton, N.J.) · 2018Article
- Rapid Generation and Detection of Biomimetic Oxygen Concentration Gradients In Vitro.Scientific reports · 2017Article
- An All-on-chip Method for Rapid Neutrophil Chemotaxis Analysis Directly from a Drop of Blood.Journal of visualized experiments : JoVE · 2017Article
- Rapid capture and labeling of cells on single domain antibodies-functionalized flow cell.Biosensors & bioelectronics · 2017Article
- Big insights from small volumes: deciphering complex leukocyte behaviors using microfluidics.Journal of leukocyte biology · 2016Review
- Microfluidic assay for precise measurements of mouse, rat, and human neutrophil chemotaxis in whole-blood droplets.Journal of leukocyte biology · 2016Article
- Graphene Oxide Nanosheets Modified with Single-Domain Antibodies for Rapid and Efficient Capture of Cells.Chemistry (Weinheim an der Bergstrasse, Germany) · 2015Article
- Whole blood human neutrophil trafficking in a microfluidic model of infection and inflammation.Lab on a chip · 2015Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Neutrophil directional migration in response to chemical gradients, also known as chemotaxis, is one of the key phenomena in the immune responses against bacterial infection. To better study neutrophils chemotaxis, several in vitro assays have been developed that replicate chemotactic gradients around neutrophils isolated from whole blood. One drawback for most of these assays is the lengthy processing of blood required for neutrophils isolation, which can alter the responsiveness of neutrophils compared to the in vivo conditions. To address this limitation, we have designed a microfluidic chip for chemotaxis studies which can use neutrophils isolated on the chip, directly from whole blood. We have tested three different cell adhesion molecules as substrates for neutrophil isolation (P-selectin, E-selectin and fibronectin) and found average capture efficiencies of 20-40 neutrophils/mm2 at optimized concentrations. Subsequent analysis of neutrophil migration in chemoattractant gradients of N-formyl-methyl-leucyl-phenylalanine (fMLP) or Interleukin-8 (IL-8) shows higher average velocities over E-selectin as compared to the P-selectin. Our microfluidic assay uses just a drop of whole blood (<10 microL) for neutrophil isolation and provides a robust platform to perform chemotaxis assays in the competing environment of different chemokines.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.