ArticlePLoS genetics2008
Indistinguishable landscapes of meiotic DNA breaks in rad50+ and rad50S strains of fission yeast revealed by a novel rad50+ recombination intermediate.
Article in PLoS genetics, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 30 citations in OpenAlex.
- Mapping Meiotic Recombination DNA Double-Strand Breaks (DSBs) Hotspots -Methodological Advances and Challenges.Advanced genetics (Hoboken, N.J.) · 2026Article
- Heterogeneous transposable elements as silencers, enhancers and targets of meiotic recombination.Chromosoma · 2019Review
- Chromatin-mediated regulators of meiotic recombination revealed by proteomics of a recombination hotspot.Epigenetics & chromatin · 2018Article
- Physical basis for long-distance communication along meiotic chromosomes.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- The histone variant H2A.Z promotes initiation of meiotic recombination in fission yeast.Nucleic acids research · 2018Article
- Quantitative Genome-Wide Measurements of Meiotic DNA Double-Strand Breaks and Protein Binding in S. pombe.Methods in molecular biology (Clifton, N.J.) · 2017Article
- Dbl2 Regulates Rad51 and DNA Joint Molecule Metabolism to Ensure Proper Meiotic Chromosome Segregation.PLoS genetics · 2016Article
- Evolutionarily diverse determinants of meiotic DNA break and recombination landscapes across the genome.Genome research · 2014Article
- Genome rearrangements and pervasive meiotic drive cause hybrid infertility in fission yeast.eLife · 2014Article
- DNA intermediates of meiotic recombination in synchronous S. pombe at optimal temperature.Nucleic acids research · 2014Article
- Acetylated Histone H3K9 is associated with meiotic recombination hotspots, and plays a role in recombination redundantly with other factors including the H3K4 methylase Set1 in fission yeast.Nucleic acids research · 2013Article
- Protein determinants of meiotic DNA break hot spots.Molecular cell · 2013Article
- New paradigms for conserved, multifactorial, cis-acting regulation of meiotic recombination.Nucleic acids research · 2012Review
- Nucleosomal organization of replication origins and meiotic recombination hotspots in fission yeast.The EMBO journal · 2012Article
- Meiotic recombination protein Rec12: functional conservation, crossover homeostasis and early crossover/non-crossover decision.Nucleic acids research · 2011Article
- Important characteristics of sequence-specific recombination hotspots in Schizosaccharomyces pombe.Genetics · 2011Article
- Article
- Meiotic DNA double-strand break repair requires two nucleases, MRN and Ctp1, to produce a single size class of Rec12 (Spo11)-oligonucleotide complexes.Molecular and cellular biology · 2009Article
- Ctp1 and the MRN-complex are required for endonucleolytic Rec12 removal with release of a single class of oligonucleotides in fission yeast.PLoS genetics · 2009Article
- Ctp1 and Exonuclease 1, alternative nucleases regulated by the MRN complex, are required for efficient meiotic recombination.Proceedings of the National Academy of Sciences of the United States of America · 2009Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
The fission yeast Schizosaccharomyces pombe Rec12 protein, the homolog of Spo11 in other organisms, initiates meiotic recombination by creating DNA double-strand breaks (DSBs) and becoming covalently linked to the DNA ends of the break. This protein-DNA linkage has previously been detected only in mutants such as rad50S in which break repair is impeded and DSBs accumulate. In the budding yeast Saccharomyces cerevisiae, the DSB distribution in a rad50S mutant is markedly different from that in wild-type (RAD50) meiosis, and it was suggested that this might also be true for other organisms. Here, we show that we can detect Rec12-DNA linkages in Sc. pombe rad50(+) cells, which are proficient for DSB repair. In contrast to the results from Sa. cerevisiae, genome-wide microarray analysis of Rec12-DNA reveals indistinguishable meiotic DSB distributions in rad50(+) and rad50S strains of Sc. pombe. These results confirm our earlier findings describing the occurrence of widely spaced DSBs primarily in large intergenic regions of DNA and demonstrate the relevance and usefulness of fission yeast studies employing rad50S. We propose that the differential behavior of rad50S strains reflects a major difference in DSB regulation between the two species--specifically, the requirement for the Rad50-containing complex for DSB formation in budding yeast but not in fission yeast. Use of rad50S and related mutations may be a useful method for DSB analysis in other species.
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