ArticleNucleic acids research2009
Evidence for RNA synthesis in the intergenic region between enhancer and promoter and its inhibition by insulators in Drosophila melanogaster.
Article in Nucleic acids research, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 25 citations in OpenAlex.
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- Strong Activation ofInternational journal of molecular sciences · 2024Article
- Genome-Wide Study of Colocalization between Genomic Stretches: A Method and Applications to the Regulation of Gene Expression.Biology · 2022Article
- Genome-wide study of correlations between genomic features and their relationship with the regulation of gene expression.DNA research : an international journal for rapid publication of reports on genes and genomes · 2015Article
- dREAM co-operates with insulator-binding proteins and regulates expression at divergently paired genes.Nucleic acids research · 2014Article
- Analysis of the enhancer-blocking function of the TBS element from Petunia hybrida in transgenic Arabidopsis thaliana and Nicotiana tabacum.Plant cell reports · 2011Article
- Enhancer-promoter interference and its prevention in transgenic plants.Plant cell reports · 2011Review
- Article
- Both the constitutive cauliflower mosaic virus 35S and tissue-specific AGAMOUS enhancers activate transcription autonomously in Arabidopsis thaliana.Plant molecular biology · 2010Article
- Actin complexes in the cell nucleus: new stones in an old field.Histochemistry and cell biology · 2010Review
- Analysis of insulator proteins binding in reporter genetic constructs transfected into Drosophila S2 cells.Doklady. Biochemistry and biophysicsArticle
- Molecular analysis of enhancer RNAs and chromatin modifications in the region of their synthesis in Drosophila cells possessing genetic constructs.Doklady. Biochemistry and biophysicsArticle
- Article
- Roles of chromatin insulator proteins in higher-order chromatin organization and transcription regulation.Nucleus (Austin, Tex.)Review
- Study of the effects of enhancer and insulator on the chromatin structure in Drosophila melanogaster.Doklady. Biochemistry and biophysicsArticle
- Molecular analysis of RNA induced by enhancer in Drosophila cells carrying reporter genetic constructs.Doklady. Biochemistry and biophysicsArticle
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Uncovering the nature of communication between enhancers, promoters and insulators is important for understanding the fundamental mechanisms that ensure appropriate gene expression levels. Here we describe an approach employing transient expression of genetic luciferase reporter gene constructs with quantitative RT-PCR analysis of transcription between an enhancer and Hsp70 promoter. We tested genetic constructs containing gypsy and/or Fab7 insulators in different orientations, and an enhancer from copia LTR-retroelement [(enh)copia]. A single gypsy or Fab7 insulator inserted between the promoter and enhancer in any polarity reduced enhancer action. A pair of insulators flanking the gene in any orientation exhibited increased insulation activity. We detected promoter-independent synthesis of non-coding RNA in the intergenic region of the constructs, which was induced by the enhancer in both directions and repressed by a single insulator or a pair of insulators. These results highlight the involvement of RNA-tracking mechanisms in the communications between enhancers and promoters, which are inhibited by insulators.
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