ArticlePurinergic signalling2009
Mouse Leydig cells express multiple P2X receptor subunits.
Article in Purinergic signalling, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 25 citations in OpenAlex.
- ATP-gated P2X7 receptor as a potential target for prostate cancer.Human cell · 2022Review
- Purinergic signaling in the male reproductive tract.Frontiers in endocrinology · 2022Review
- Article
- Sex-Related Overactivation of NLRP3 Inflammasome Increases Lethality of the Male COVID-19 Patients.Frontiers in molecular biosciences · 2021Review
- Nitric oxide modulates ATP-evoked currents in mouse Leydig cells.Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologica · 2018Article
- Purinergic signaling in testes revealed.The Journal of general physiology · 2016Article
- Distinct purinergic signaling pathways in prepubescent mouse spermatogonia.The Journal of general physiology · 2016Article
- Purinergic signaling pathways in endocrine system.Autonomic neuroscience : basic & clinical · 2015Review
- Purinergic signalling in the reproductive system in health and disease.Purinergic signalling · 2014Review
- Purinergic signalling in endocrine organs.Purinergic signalling · 2014Review
- Porphyromonas gingivalis fimbriae dampen P2X7-dependent interleukin-1β secretion.Journal of innate immunity · 2014Article
- P2X receptor channels in endocrine glands.Wiley interdisciplinary reviews. Membrane transport and signaling · 2013Article
- ATP receptors gate microglia signaling in neuropathic pain.Experimental neurology · 2012Review
- Interaction of purinergic receptors with GPCRs, ion channels, tyrosine kinase and steroid hormone receptors orchestrates cell function.Purinergic signalling · 2012Article
- Activation and regulation of purinergic P2X receptor channels.Pharmacological reviews · 2011Review
- P2X4 receptors interact with both P2X2 and P2X7 receptors in the form of homotrimers.British journal of pharmacology · 2011Article
- High expression and activity of ecto-5'-nucleotidase/CD73 in the male murine reproductive tract.Histochemistry and cell biology · 2010Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
ATP acts on cellular membranes by interacting with P2X (ionotropic) and P2Y (metabotropic) receptors. Seven homomeric P2X receptors (P2X(1)-P2X(7)) and seven heteromeric receptors (P2X(1/2), P2X(1/4), P2X(1/5), P2X(2/3), P2X(2/6), P2X(4/6), P2X(4/7)) have been described. ATP treatment of Leydig cells leads to an increase in [Ca(2+)](i) and testosterone secretion, supporting the hypothesis that Ca(2+) signaling through purinergic receptors contributes to the process of testosterone secretion in these cells. Mouse Leydig cells have P2X receptors with a pharmacological and biophysical profile resembling P2X(2). In this work, we describe the presence of several P2X receptor subunits in mouse Leydig cells. Western blot experiments showed the presence of P2X(2), P2X(4), P2X(6), and P2X(7) subunits. These results were confirmed by immunofluorescence. Functional results support the hypothesis that heteromeric receptors are present in these cells since 0.5 muM ivermectin induced an increase (131.2 +/- 5.9%) and 3 muM ivermectin a decrease (64.2 +/- 4.8%) in the whole-cell currents evoked by ATP. These results indicate the presence of functional P2X(4) subunits. P2X(7) receptors were also present, but they were non-functional under the present conditions because dye uptake experiments with Lucifer yellow and ethidium bromide were negative. We conclude that a heteromeric channel, possibly P2X(2/4/6), is present in Leydig cells, but with an electrophysiological and pharmacological phenotype characteristic of the P2X(2) subunit.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.