ArticleMethods in molecular biology (Clifton, N.J.)2009
Multiparameter flow cytometry monitoring of T cell responses.
Article in Methods in molecular biology (Clifton, N.J.), 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
24 citing papers in PubMed.
- A randomized, controlled dose-finding Phase II study of the M72/AS01 candidate tuberculosis vaccine in healthy PPD-positive adults.Journal of clinical immunology · 2013Trial
- Trial
- H5N1 influenza vaccine formulated with AS03 A induces strong cross-reactive and polyfunctional CD4 T-cell responses.Journal of clinical immunology · 2011Trial
- Article
- The Biodegradability andFrontiers in bioengineering and biotechnology · 2022Article
- Article
- Macrophage and dendritic cell subset composition can distinguish endotypes in adjuvant-induced asthma mouse models.PloS one · 2021Article
- Human T-Cell Cloning by Limiting Dilution.Methods in molecular biology (Clifton, N.J.) · 2021Article
- The use of cell-mediated immunity for the evaluation of influenza vaccines: an upcoming necessity.Human vaccines & immunotherapeutics · 2019Review
- Improved Detection of Cytokines Produced by Invariant NKT Cells.Scientific reports · 2017Article
- Isolation, Cryopreservation, and Immunophenotyping of Human Peripheral Blood Mononuclear Cells.Current protocols in toxicology · 2017Article
- Direct TLR-2 Costimulation Unmasks the Proinflammatory Potential of Neonatal CD4+ T Cells.Journal of immunology (Baltimore, Md. : 1950) · 2016Article
- Review
- Intracellular Cytokine Staining on PBMCs Using CyTOF™ Mass Cytometry.Bio-protocol · 2015Article
- Multiparameter Phenotyping of Human PBMCs Using Mass Cytometry.Methods in molecular biology (Clifton, N.J.) · 2015Article
- Monitoring the immune competence of cancer patients to predict outcome.Cancer immunology, immunotherapy : CII · 2014Review
- Functionality of CD4+ and CD8+ T cells from tonsillar tissue.Clinical and experimental immunology · 2012Article
- Dynamics of cell-mediated immune responses to cytomegalovirus in pediatric transplantation recipients.Pediatric transplantation · 2012Article
- Biomarkers in T cell therapy clinical trials.Journal of translational medicine · 2011Review
- Multiparameter intracellular cytokine staining.Methods in molecular biology (Clifton, N.J.) · 2011Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
1 author.
Funding
Abstract
HIV vaccine research increasingly uses polychromatic flow cytometry as a tool to monitor T cell responses. The use of this technology allows for the analysis of highly defined subsets of cells with unique phenotypes and functions. Ultimately, such studies may identify surrogate markers of protection from disease progression. However, this powerful technology comes with a number of technical hurdles, and there is a need to standardize the assays and protocols used in clinical trial monitoring. Here an optimized protocol, with variations for specific circumstances, is presented. This protocol covers the analysis of multiple cytokines, cell surface markers, and other functional markers such as perforin, CD107, and CD154. While the protocol can be adapted to various numbers of fluorescence parameters, optimized panels of 8-10 colors are presented.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.