ArticleActa crystallographica. Section D, Biological crystallography2008
The plug-based nanovolume Microcapillary Protein Crystallization System (MPCS).
Article in Acta crystallographica. Section D, Biological crystallography, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
25 citing papers in PubMed.
- Optimization of crystallization of biological macromolecules using dialysis combined with temperature control.Journal of applied crystallography · 2020Article
- Graphene-based microfluidics for serial crystallography.Lab on a chip · 2016Article
- Capture and X-ray diffraction studies of protein microcrystals in a microfluidic trap array.Acta crystallographica. Section D, Biological crystallography · 2015Article
- Cleaning protocols for crystallization robots: preventing protease contamination.Acta crystallographica. Section F, Structural biology communications · 2015Article
- X-ray Transparent Microfluidic Chip for Mesophase-Based Crystallization of Membrane Proteins and On-Chip Structure Determination.Crystal growth & design · 2014Article
- Carboxylic acids in crystallization of macromolecules: learning from successful crystallization experiments.Journal of structural and functional genomics · 2014Article
- Approaches to automated protein crystal harvesting.Acta crystallographica. Section F, Structural biology communications · 2014Article
- An X-ray transparent microfluidic platform for screening of the phase behavior of lipidic mesophases.The Analyst · 2013Article
- Acoustic methods for high-throughput protein crystal mounting at next-generation macromolecular crystallographic beamlines.Journal of synchrotron radiation · 2013Article
- A microfluidic approach for protein structure determination at room temperature via on-chip anomalous diffraction.Lab on a chip · 2013Article
- Article
- Microfluidic devices: useful tools for bioprocess intensification.Molecules (Basel, Switzerland) · 2011Review
- Salvage and storage of infectious disease protein targets in the SSGCID high-throughput crystallization pathway using microfluidics.Acta crystallographica. Section F, Structural biology and crystallization communications · 2011Article
- Structural genomics of infectious disease drug targets: the SSGCID.Acta crystallographica. Section F, Structural biology and crystallization communications · 2011Article
- Inhibitor-bound complexes of dihydrofolate reductase-thymidylate synthase from Babesia bovis.Acta crystallographica. Section F, Structural biology and crystallization communications · 2011Article
- Wheat germ cell-free expression system as a pathway to improve protein yield and solubility for the SSGCID pipeline.Acta crystallographica. Section F, Structural biology and crystallization communications · 2011Article
- SAD phasing using iodide ions in a high-throughput structural genomics environment.Journal of structural and functional genomics · 2011Article
- Development of high-performance X-ray transparent crystallization plates for in situ protein crystal screening and analysis.Acta crystallographica. Section D, Biological crystallography · 2011Article
- Unmet challenges of structural genomics.Current opinion in structural biology · 2010Review
- Nanovolume optimization of protein crystal growth using the microcapillary protein crystallization system.Journal of applied crystallography · 2010Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors.
Funding
Abstract
The Microcapillary Protein Crystallization System (MPCS) embodies a new semi-automated plug-based crystallization technology which enables nanolitre-volume screening of crystallization conditions in a plasticware format that allows crystals to be easily removed for traditional cryoprotection and X-ray diffraction data collection. Protein crystals grown in these plastic devices can be directly subjected to in situ X-ray diffraction studies. The MPCS integrates the formulation of crystallization cocktails with the preparation of the crystallization experiments. Within microfluidic Teflon tubing or the microfluidic circuitry of a plastic CrystalCard, approximately 10-20 nl volume droplets are generated, each representing a microbatch-style crystallization experiment with a different chemical composition. The entire protein sample is utilized in crystallization experiments. Sparse-matrix screening and chemical gradient screening can be combined in one comprehensive ;hybrid' crystallization trial. The technology lends itself well to optimization by high-granularity gradient screening using optimization reagents such as precipitation agents, ligands or cryoprotectants.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.