ArticleJournal of clinical microbiology2009
Development of an oligonucleotide array for direct detection of fungi in sputum samples from patients with cystic fibrosis.
Article in Journal of clinical microbiology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 73 citations in OpenAlex.
- Diagnosis and management of invasive fungal infections due to non-Aspergillus moulds.The Journal of antimicrobial chemotherapy · 2025Review
- PCR-Based Microarray Enhances Diagnosis of Culture-Negative Biopsied Tissue in Patients with Invasive Mold Infections: Real-World Experience in a Tertiary Medical Center.Journal of fungi (Basel, Switzerland) · 2024Article
- Upper respiratory tract mycobiome alterations in different kinds of pulmonary disease.Frontiers in microbiology · 2023Article
- Review
- Diagnosing Fungal Keratitis and Simultaneously IdentifyingJournal of fungi (Basel, Switzerland) · 2022Article
- Review
- Fungal Pathogens in CF Airways: Leave or Treat?Mycopathologia · 2018Review
- Challenges in Laboratory Detection of Fungal Pathogens in the Airways of Cystic Fibrosis Patients.Mycopathologia · 2018Article
- Article
- Purification and characterization of a mycelial catalase from Scedosporium boydii, a useful tool for specific antibody detection in patients with cystic fibrosis.Clinical and vaccine immunology : CVI · 2015Article
- Rapid detection of dermatophytes and Candida albicans in onychomycosis specimens by an oligonucleotide array.BMC infectious diseases · 2014Article
- Article
- Article
- Exploring the parallel development of microbial systems in neonates with cystic fibrosis.mBio · 2012Article
- Clinical and microbiological efficacy of micafungin on Geosmithia argillacea infection in a cystic fibrosis patient.Medical mycology case reports · 2012Article
- The airway microbiota in cystic fibrosis: a complex fungal and bacterial community--implications for therapeutic management.PloS one · 2012Article
- Article
- Phenazine-1-carboxylic acid promotes bacterial biofilm development via ferrous iron acquisition.Journal of bacteriology · 2011Article
- Aspergillus species and other molds in respiratory samples from patients with cystic fibrosis: a laboratory-based study with focus on Aspergillus fumigatus azole resistance.Journal of clinical microbiology · 2011Article
- Rapid identification of allergenic and pathogenic molds in environmental air by an oligonucleotide array.BMC infectious diseases · 2011Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cystic fibrosis (CF) is the most common inherited genetic disease in Caucasian populations. Besides bacteria, many species of fungi may colonize the respiratory tract of these patients, sometimes leading to true respiratory infections. In this study, an oligonucleotide array capable of identifying 20 fungal species was developed to directly detect fungi in the sputum samples of CF patients. Species-specific oligonucleotide probes were designed from the internal transcribed spacer (ITS) regions of the rRNA operon and immobilized on a nylon membrane. The fungal ITS regions were amplified by PCR and hybridized to the array for species identification. The array was validated by testing 182 target strains (strains which we aimed to identify) and 141 nontarget strains (135 species), and a sensitivity of 100% and a specificity of 99.2% were obtained. The validated array was then used for direct detection of fungi in 57 sputum samples from 39 CF patients, and the results were compared to those obtained by culture. For 16 sputum samples, the results obtained by the array corresponded with those obtained by culture. For 33 samples, the array detected more fungal species than culture did, while the reverse was found for eight samples. The accuracy of the array for fungal detection in sputum samples was confirmed (or partially confirmed) in some samples by cloning and resequencing the amplified ITS fragments. The present array is a useful tool for both the simultaneous detection of multiple fungal species present in the sputa of CF patients and the identification of fungi isolated from these patients.
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