ArticleThe Journal of neuroscience : the official journal of the Society for Neuroscience2008
Sec24- and ARFGAP1-dependent trafficking of GABA transporter-1 is a prerequisite for correct axonal targeting.
Article in The Journal of neuroscience : the official journal of the Society for Neuroscience, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
24 citing papers in PubMed, 35 citations in OpenAlex.
- Diacidic Motifs in the Carboxyl Terminus Are Required for ER Exit and Translocation to the Plasma Membrane of NKCC2.International journal of molecular sciences · 2022Article
- Functional and Biochemical Consequences of Disease Variants in Neurotransmitter Transporters: A Special Emphasis on Folding and Trafficking Deficits.Pharmacology & therapeutics · 2021Review
- Constitutive Endocytosis of the Neuronal Glutamate Transporter Excitatory Amino Acid Transporter-3 Requires ARFGAP1.Frontiers in physiology · 2021Article
- Review
- Identification and characterization of the Fasciola hepatica sodium- and chloride-dependent taurine transporter.PLoS neglected tropical diseases · 2018Article
- High abundance of ArfGAP1 found in the mossy fibers in hilus of the dentate gyrus region of the mouse brain.PloS one · 2017Article
- When transporters fail to be transported: how to rescue folding-deficient SLC6 transporters.Journal of neurology & neuromedicine · 2016Article
- Regulation of EGFR surface levels by COPII-dependent trafficking.The Journal of cell biology · 2016Article
- Functional Rescue of a MisfoldedThe Journal of biological chemistry · 2016Article
- A salt bridge linking the first intracellular loop with the C terminus facilitates the folding of the serotonin transporter.The Journal of biological chemistry · 2015Article
- Axonal targeting of the serotonin transporter in cultured rat dorsal raphe neurons is specified by SEC24C-dependent export from the endoplasmic reticulum.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2014Article
- DAWN: a framework to identify autism genes and subnetworks using gene expression and genetics.Molecular autism · 2014Article
- Regulation of traffic and organelle architecture of the ER-Golgi interface by signal transduction.Histochemistry and cell biology · 2013Review
- Switching the clientele: a lysine residing in the C terminus of the serotonin transporter specifies its preference for the coat protein complex II component SEC24C.The Journal of biological chemistry · 2013Article
- Multiple evolutionarily conserved Di-leucine like motifs in the carboxyl terminus control the anterograde trafficking of NKCC2.The Journal of biological chemistry · 2012Article
- The serotonin transporter is an exclusive client of the coat protein complex II (COPII) component SEC24C.The Journal of biological chemistry · 2011Article
- High-resolution simultaneous voltage and Ca2+ imaging.The Journal of physiology · 2011Article
- Mutations in the carboxyl-terminal SEC24 binding motif of the serotonin transporter impair folding of the transporter.The Journal of biological chemistry · 2010Article
- The N terminus of monoamine transporters is a lever required for the action of amphetamines.The Journal of biological chemistry · 2010Article
- The reverse operation of Na(+)/Cl(-)-coupled neurotransmitter transporters--why amphetamines take two to tango.Journal of neurochemistry · 2010Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 2 institutions in 2 countries.
Funding
Abstract
The GABA transporter-1 (GAT1) is a prototypical protein of the synaptic specialization. Export of GAT1 from the endoplasmic reticulum (ER) is contingent on its interaction with the COPII (coatomer protein-II) coat subunit Sec24D. Here we show that silencing all four Sec24 isoforms strongly inhibits transport of GAT1 to the cell surface. In contrast, transport of GAT1-RL/AS, a mutant that is deficient in Sec24D recruitment, was not inhibited, suggesting a nonconventional, COPII-independent pathway. However, ARFGAP1 bound directly to the C terminus of both GAT1-RL/AS and wild-type GAT1. Surface expression of GAT1-RL/AS involved ARFGAP1. GAT1-RL/AS appeared to bypass the ER-Golgi-intermediate compartment, but its pathway to the plasma membrane still involved passage through the Golgi. Thus, the GAT1-RL/AS mutant allowed to test whether COPII-dependent ER-export is required for correct sorting of GAT1 to the axon terminal in neuronal cells. In contrast to wild-type GAT1, GAT1-RL/AS failed to be specifically enriched at the tip of neurite extensions of CAD.a cells (a neuroblastoma cell line that can be differentiated into a neuron-like phenotype) and in the axon terminals of hippocampal neurons. These findings indicate that correct sorting to the axon is contingent on ER export via the COPII machinery and passage through the ER-Golgi-intermediate compartment.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.