ArticleMolecular biology of the cell2009
Mutations in the Arabidopsis peroxisomal ABC transporter COMATOSE allow differentiation between multiple functions in planta: insights from an allelic series.
Article in Molecular biology of the cell, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 39 citations in OpenAlex.
- Hierarchical regulation of oxygen-sensing through coupled transducers in Arabidopsis thaliana.Nature communications · 2026Article
- Structural insights into Arabidopsis thaliana CTS-mediated fatty acid and hormone metabolism.Nature communications · 2026Article
- Predictive modelling of the COMATOSE transporter reveals a conserved ligand binding pocket for acyl-CoAs.Scientific reports · 2026Article
- ERFVII action and modulation through oxygen-sensing in Arabidopsis thaliana.Nature communications · 2023Article
- Mitochondrial retrograde signaling through UCP1-mediated inhibition of the plant oxygen-sensing pathway.Current biology : CB · 2022Article
- Mutagenesis separates ATPase and thioesterase activities of the peroxisomal ABC transporter, Comatose.Scientific reports · 2019Article
- Plant peroxisomal solute transporter proteins.Journal of integrative plant biology · 2019Review
- Autophagy regulates glucose-mediated root meristem activity by modulating ROS production in Arabidopsis.Autophagy · 2019Article
- Genetic interactions between ABA signalling and the Arg/N-end rule pathway during Arabidopsis seedling establishment.Scientific reports · 2018Article
- Genome-Wide Identification of the MIKC-Type MADS-Box Gene Family inFrontiers in plant science · 2017Article
- The Roles of β-Oxidation and Cofactor Homeostasis in Peroxisome Distribution and Function in Arabidopsis thaliana.Genetics · 2016Article
- How to move an amphipathic molecule across a lipid bilayer: different mechanisms for different ABC transporters?Biochemical Society transactions · 2016Review
- Peroxisomal ABC transporters: functions and mechanism.Biochemical Society transactions · 2015Review
- In vitro reassembly of the ribose ATP-binding cassette transporter reveals a distinct set of transport complexes.The Journal of biological chemistry · 2015Article
- Barley has two peroxisomal ABC transporters with multiple functions in β-oxidation.Journal of experimental botany · 2014Article
- Peroxisomal ATP-binding cassette transporter COMATOSE and the multifunctional protein abnormal INFLORESCENCE MERISTEM are required for the production of benzoylated metabolites in Arabidopsis seeds.Plant physiology · 2014Article
- The α/β hydrolase CGI-58 and peroxisomal transport protein PXA1 coregulate lipid homeostasis and signaling in Arabidopsis.The Plant cell · 2013Article
- Intrinsic acyl-CoA thioesterase activity of a peroxisomal ATP binding cassette transporter is required for transport and metabolism of fatty acids.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Acyl-lipid metabolism.The arabidopsis book · 2013Article
- Individual maize chromosomes in the C(3) plant oat can increase bundle sheath cell size and vein density.Plant physiology · 2012Article
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Authors and funding
8 authors at 3 institutions in 1 country.
Funding
Abstract
COMATOSE (CTS), the Arabidopsis homologue of human Adrenoleukodystrophy protein (ALDP), is required for import of substrates for peroxisomal beta-oxidation. A new allelic series and a homology model based on the bacterial ABC transporter, Sav1866, provide novel insights into structure-function relations of ABC subfamily D proteins. In contrast to ALDP, where the majority of mutations result in protein absence from the peroxisomal membrane, all CTS mutants produced stable protein. Mutation of conserved residues in the Walker A and B motifs in CTS nucleotide-binding domain (NBD) 1 resulted in a null phenotype but had little effect in NBD2, indicating that the NBDs are functionally distinct in vivo. Two alleles containing mutations in NBD1 outside the Walker motifs (E617K and C631Y) exhibited resistance to auxin precursors 2,4-dichlorophenoxybutyric acid (2,4-DB) and indole butyric acid (IBA) but were wild type in all other tests. The homology model predicted that the transmission interfaces are domain-swapped in CTS, and the differential effects of mutations in the conserved "EAA motif" of coupling helix 2 supported this prediction, consistent with distinct roles for each NBD. Our findings demonstrate that CTS functions can be separated by mutagenesis and the structural model provides a framework for interpretation of phenotypic data.
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