ArticleThe Journal of biological chemistry2009
Structure and functional studies of the CS domain of the essential H/ACA ribonucleoparticle assembly protein SHQ1.
Article in The Journal of biological chemistry, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 28 citations in OpenAlex.
- Biallelic SHQ1 variants in early infantile hypotonia and paroxysmal dystonia as the leading manifestation.Human genetics · 2023Article
- Crystal structures of the naturally fused CS and cytochrome bActa crystallographica. Section D, Structural biology · 2019Article
- Assembly and trafficking of box C/D and H/ACA snoRNPs.RNA biology · 2017Review
- Structure and interactions of the CS domain of human H/ACA RNP assembly protein Shq1.Journal of molecular biology · 2015Article
- RNA-guided isomerization of uridine to pseudouridine--pseudouridylation.RNA biology · 2014Review
- Mechanism of the AAA+ ATPases pontin and reptin in the biogenesis of H/ACA RNPs.RNA (New York, N.Y.) · 2012Article
- Structure of the Shq1-Cbf5-Nop10-Gar1 complex and implications for H/ACA RNP biogenesis and dyskeratosis congenita.The EMBO journal · 2011Article
- The H/ACA RNP assembly factor SHQ1 functions as an RNA mimic.Genes & development · 2011Article
- Structural features and chaperone activity of the NudC protein family.Journal of molecular biology · 2011Article
- Structures of ribonucleoprotein particle modification enzymes.Quarterly reviews of biophysics · 2011Review
- GRIM-1, a novel growth suppressor, inhibits rRNA maturation by suppressing small nucleolar RNAs.PloS one · 2011Article
- Rapid global structure determination of large RNA and RNA complexes using NMR and small-angle X-ray scattering.Methods (San Diego, Calif.) · 2010Review
- Integrative genomic profiling of human prostate cancer.Cancer cell · 2010Article
- Pathogenic NAP57 mutations decrease ribonucleoprotein assembly in dyskeratosis congenita.Human molecular genetics · 2009Article
- The box H/ACA snoRNP assembly factor Shq1p is a chaperone protein homologous to Hsp90 cochaperones that binds to the Cbf5p enzyme.Journal of molecular biology · 2009Article
- SHQ1 is required prior to NAF1 for assembly of H/ACA small nucleolar and telomerase RNPs.RNA (New York, N.Y.) · 2009Article
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
H/ACA ribonucleoprotein particles are essential for ribosomal RNA and telomerase RNA processing and metabolism. Shq1p has been identified as an essential eukaryotic H/ACA small nucleolar (sno) ribonucleoparticle (snoRNP) biogenesis and assembly factor. Shq1p is postulated to be involved in the early biogenesis steps of H/ACA snoRNP complexes, and Shq1p depletion leads to a specific decrease in H/ACA small nucleolar RNA levels and to defects in ribosomal RNA processing. Shq1p contains two predicted domains as follows: an N-terminal CS (named after CHORD-containing proteins and SGT1) or HSP20-like domain, and a C-terminal region of high sequence homology called the Shq1 domain. Here we report the crystal structure and functional studies of the Saccharomyces cerevisiae Shq1p CS domain. The structure consists of a compact anti-parallel beta-sandwich fold that is composed of two beta-sheets containing four and three beta-strands, respectively, and a short alpha-helix. Deletion studies showed that the CS domain is required for the essential functions of Shq1p. Point mutations in residues Phe-6, Gln-10, and Lys-80 destabilize Shq1p in vivo and induce a temperature-sensitive phenotype with depletion of H/ACA small nucleolar RNAs and defects in rRNA processing. Although CS domains are frequently found in co-chaperones of the Hsp90 molecular chaperone, no interaction was detected between the Shq1p CS domain and yeast Hsp90 in vitro. These results show that the CS domain is essential for Shq1p function in H/ACA snoRNP biogenesis in vivo, possibly in an Hsp90-independent manner.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.