ArticleMolecular microbiology2008
A single aromatic residue in transcriptional repressor protein KorA is critical for cooperativity with its co-regulator KorB.
Article in Molecular microbiology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
10 citing papers in PubMed, 16 citations in OpenAlex.
- Evolution of a Plasmid Regulatory Circuit Ameliorates Plasmid Fitness Cost.Molecular biology and evolution · 2025Article
- KorB switching from DNA-sliding clamp to repressor mediates long-range gene silencing in a multi-drug resistance plasmid.Nature microbiology · 2025Article
- Evolution of a Plasmid Regulatory Circuit Ameliorates Plasmid Fitness Cost.bioRxiv : the preprint server for biology · 2024Article
- Intrinsic disorder in the partitioning protein KorB persists after co-operative complex formation with operator DNA and KorA.The Biochemical journal · 2017Article
- Flexibility of KorA, a plasmid-encoded, global transcription regulator, in the presence and the absence of its operator.Nucleic acids research · 2016Article
- Global Transcriptional Regulation of Backbone Genes in Broad-Host-Range Plasmid RA3 from the IncU Group Involves Segregation Protein KorB (ParB Family).Applied and environmental microbiology · 2016Article
- Negative feedback and transcriptional overshooting in a regulatory network for horizontal gene transfer.PLoS genetics · 2014Article
- Adaptation for protein synthesis efficiency in a naturally occurring self-regulating operon.PloS one · 2012Article
- Global transcription regulation of RK2 plasmids: a case study in the combined use of dynamical mathematical models and statistical inference for integration of experimental data and hypothesis exploration.BMC systems biology · 2011Article
- Crystal structure of KorA bound to operator DNA: insight into repressor cooperation in RP4 gene regulation.Nucleic acids research · 2009Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
A central feature of broad host range IncP-1 plasmids is the set of regulatory circuits that tightly control plasmid core functions under steady-state conditions. Cooperativity between KorB and either KorA or TrbA repressor proteins is a key element of these circuits and deletion analysis has implicated the conserved C-terminal domain of KorA and TrbA in this interaction. By NMR we show that KorA and KorB interact directly and identify KorA amino acids that are affected on KorB binding. Studies on mutants showed that tyrosine 84 (or phenylalanine, in some alleles) is dispensable for repressor activity but critical for the specific interaction with KorB in both in vivo reporter gene assays and in vitro electrophoretic mobility shift and co-purification assays. This confirms that direct and specific protein-protein interactions are responsible for the cooperativity observed between KorB and its corepressors and lays the basis for determining the biological importance of this cooperativity.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.