ArticleMolecular microbiology2008
Abrogation of ospAB constitutively activates the Rrp2-RpoN-RpoS pathway (sigmaN-sigmaS cascade) in Borrelia burgdorferi.
Article in Molecular microbiology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 36 citations in OpenAlex.
- Article
- Article
- Positive feedback regulation between RpoS and BosR in the Lyme disease pathogen.bioRxiv : the preprint server for biology · 2024Article
- A systemic approach to identify non-abundant immunogenic proteins in Lyme disease pathogens.mSystems · 2024Article
- The Rrp2-RpoN-RpoS pathway plays an important role in the blood-brain barrier transmigration of the Lyme disease pathogen.Infection and immunity · 2023Article
- Gene Regulation and Transcriptomics.Current issues in molecular biology · 2021Article
- Tracking ofInfection and immunity · 2019Article
- Borrelia Host Adaptation Protein (BadP) Is Required for the Colonization of a Mammalian Host by the Agent of Lyme Disease.Infection and immunity · 2018Article
- Investigation ofFrontiers in cellular and infection microbiology · 2017Article
- Outer surface protein OspC is an antiphagocytic factor that protects Borrelia burgdorferi from phagocytosis by macrophages.Infection and immunity · 2015Article
- Article
- DhhP, a cyclic di-AMP phosphodiesterase of Borrelia burgdorferi, is essential for cell growth and virulence.Infection and immunity · 2014Article
- Article
- Synthesis of RpoS is dependent on a putative enhancer binding protein Rrp2 in Borrelia burgdorferi.PloS one · 2014Article
- Borrelia burgdorferi oxidative stress regulator BosR directly represses lipoproteins primarily expressed in the tick during mammalian infection.Molecular microbiology · 2013Article
- Contributions of environmental signals and conserved residues to the functions of carbon storage regulator A of Borrelia burgdorferi.Infection and immunity · 2013Article
- Manganese and zinc regulate virulence determinants in Borrelia burgdorferi.Infection and immunity · 2013Article
- Genome stability of Lyme disease spirochetes: comparative genomics of Borrelia burgdorferi plasmids.PloS one · 2012Article
- Regulation of the virulence determinant OspC by bbd18 on linear plasmid lp17 of Borrelia burgdorferi.Journal of bacteriology · 2011Article
- Oligopeptide permease A5 modulates vertebrate host-specific adaptation of Borrelia burgdorferi.Infection and immunity · 2011Article
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
Molecular mechanisms underlying the reciprocal regulation of the two major surface lipoproteins and virulence factors of Borrelia burgdorferi, OspA and OspC, are not fully understood. Herein, we report that inactivation of the ospAB operon resulted in overproduction of OspC and many other lipoproteins via the constitutive activation of the Rrp2-RpoN-RpoS pathway. Complementing the ospAB mutant with a wild-type copy of ospA, but not an ospA variant that lacks the lipoprotein signal sequence, restored normal regulation of the Rrp2-RpoN-RpoS pathway; these results indicate that the phenotype was not caused by spurious mutations. Interestingly, while most of the ospAB mutant clones displayed a constitutive ospC expression phenotype, some ospAB mutant clones showed little or no ospC expression. Further analyses revealed that this OspC-negative phenotype was independent of abrogation of ospAB. While activation of the Rrp2-RpoN-RpoS pathway was recently shown to downregulate ospA, our findings suggest that reduction of OspA can also activate this pathway. We postulate that the activation of the Rrp2-RpoN-RpoS pathway and downregulation of OspA form a positive feedback loop that allows spirochaetes to produce and maintain a constant high level of OspC and other lipoproteins during tick feeding, a strategy that is critical for spirochaetal transmission and mammalian infection.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.