ArticleMolecular microbiology2009
DNA polymerase switching: effects on spontaneous mutagenesis in Escherichia coli.
Article in Molecular microbiology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
36 citing papers in PubMed, 52 citations in OpenAlex.
- Mutations in ribosomal protein uS5 alter translation fidelity and mutagenesis inJournal of bacteriology · 2025Article
- Escherichia coli DNA replication: the old model organism still holds many surprises.FEMS microbiology reviews · 2024Review
- Environmental and genetic influence on the rate and spectrum of spontaneous mutations inMicrobiology (Reading, England) · 2024Article
- Strand specificity of ribonucleotide excision repair in Escherichia coli.Nucleic acids research · 2023Article
- Novel Escherichia coli active site dnaE alleles with altered base and sugar selectivity.Molecular microbiology · 2021Article
- Effect of mismatch repair on the mutational footprint of the bacterial SOS mutator activity.DNA repair · 2021Article
- New complexities of SOS-induced "untargeted" mutagenesis in Escherichia coli as revealed by mutation accumulation and whole-genome sequencing.DNA repair · 2020Article
- Role of RNase H enzymes in maintaining genome stability in Escherichia coli expressing a steric-gate mutant of pol VDNA repair · 2019Article
- The SOS system: A complex and tightly regulated response to DNA damage.Environmental and molecular mutagenesis · 2019Review
- SetRDNA repair · 2019Article
- Specificity of the DNA Mismatch Repair System (MMR) and Mutagenesis Bias in Bacteria.Molecular biology and evolution · 2018Article
- Sources of spontaneous mutagenesis in bacteria.Critical reviews in biochemistry and molecular biology · 2018Review
- Fluorescence-Based Reporters for Detection of Mutagenesis in E. coli.Methods in enzymology · 2017Article
- Antibiotic treatment enhances the genome-wide mutation rate of target cells.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Deletion of the 2-acyl-glycerophosphoethanolamine cycle improve glucose metabolism in Escherichia coli strains employed for overproduction of aromatic compounds.Microbial cell factories · 2015Article
- Determinants of spontaneous mutation in the bacterium Escherichia coli as revealed by whole-genome sequencing.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Investigating the mechanisms of ribonucleotide excision repair in Escherichia coli.Mutation research · 2014Article
- Hypermutability and error catastrophe due to defects in ribonucleotide reductase.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Removal of misincorporated ribonucleotides from prokaryotic genomes: an unexpected role for nucleotide excision repair.PLoS genetics · 2013Article
- DNA repair in Mycoplasma gallisepticum.BMC genomics · 2013Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Escherichia coli possesses five known DNA polymerases (pols). Pol III holoenzyme is the cell's main replicase, while pol I is responsible for the maturation of Okazaki fragments and filling gaps generated during nucleotide excision repair. Pols II, IV and V are significantly upregulated as part of the cell's global SOS response to DNA damage and under these conditions, may alter the fidelity of DNA replication by potentially interfering with the ability of pols I and III to complete their cellular functions. To test this hypothesis, we determined the spectrum of rpoB mutations arising in an isogenic set of mutL strains differentially expressing the chromosomally encoded pols. Interestingly, mutagenic hot spots in rpoB were identified that are susceptible to the actions of pols I-V. For example, in a recA730 lexA(Def) mutL background most transversions were dependent upon pols IV and V. In contrast, transitions were largely dependent upon pol I and to a lesser extent, pol III. Furthermore, the extent of pol I-dependent mutagenesis at one particular site was modulated by pols II and IV. Our observations suggest that there is considerable interplay among all five E. coli polymerases that either reduces or enhances the mutagenic load on the E. coli chromosome.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.