ArticleMolecular microbiology2008
Identification of VceA and VceC, two members of the VjbR regulon that are translocated into macrophages by the Brucella type IV secretion system.
Article in Molecular microbiology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 120 papers.
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120 citing papers in PubMed, 197 citations in OpenAlex.
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- Effect of Brucella effector protein BtpB regulating apoptosis by targeting host protein YWHAZ.Veterinary research · 2026Article
- Genome-wide identification of metabolic and regulatory determinants of intracellular growth inbioRxiv : the preprint server for biology · 2026Article
- Nuclear Localization of Effector BPE159: A Pivotal Mechanism for Intracellular Persistence ofMicroorganisms · 2026Article
- A subset of type 4 secretion system effectors ofmicroLife · 2026Article
- Transmembrane effector substrates of type IV secretion systems: mechanisms of secretion and insertion into host cell membranes.microLife · 2026Review
- The atypical quorum sensing system of classicalJournal of bacteriology · 2025Review
- The synergistic pathogenic mechanism ofJournal of medical microbiology · 2025Article
- Deciphering macrophage dynamics and immune evasion strategies by the intracellular bacteria.Immunologic research · 2025Review
- Breaking the silence-role of MucR as a virulence determinant inJournal of bacteriology · 2025Review
- ThemicroLife · 2025Review
- Identification of Brucella RS15060 as a novel type IV secretion system effector associated with bacterial virulence.Veterinary research · 2024Article
- Copper sensing transcription factor ArsR2 regulates VjbR to sustain virulence inEmerging microbes & infections · 2024Article
- The T4bSS of Legionella features a two-step secretion pathway with an inner membrane intermediate for secretion of transmembrane effectors.PLoS pathogens · 2024Article
- Gut epithelial electrical cues drive differential localization of enterobacteria.Nature microbiology · 2024Article
- Review
- TheFrontiers in microbiology · 2023Article
- Inflammatory Mechanism ofInternational journal of molecular sciences · 2022Review
- Brucella ceti and Brucella pinnipedialis genome characterization unveils genetic features that highlight their zoonotic potential.MicrobiologyOpen · 2022Article
- Antibiotic persistence of intracellular Brucella abortus.PLoS neglected tropical diseases · 2022Article
60 more citing papers are in PubMed but not listed here.
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5 authors at 1 institution in 1 country.
Funding
Abstract
Survival and replication inside host cells by Brucella spp. requires a type IV secretion system (T4SS), encoded by the virB locus. However, the identity of the molecules secreted by the T4SS has remained elusive. We hypothesized that proteins translocated by the T4SS would be co-regulated with the virB operon. The LuxR family regulator VjbR, known to regulate virB, bound a fragment of the virB promoter containing an 18 bp palindromic motif (virB promoter box), showing that VjbR regulated the virB operon directly. To identify virB co-regulated genes, we searched the Brucella suis 1330 and B. abortus 2308 genomes for genes with an upstream virB promoter box. One hundred and forty-four promoters in the two genomes contained the virB promoter box, including those of fliC encoding flagellin and cgs encoding cyclic beta-glucan synthetase. Thirteen of these proteins were tested for VirB-dependent translocation into macrophages using a beta-lactamase reporter assay. This analysis resulted in the identification of the proteins encoded by BAB1_1652 (VceA) and BR1038/BAB1_1058 (VceC) as novel protein substrates of the Brucella T4SS. VceC could also be translocated by the Legionella pneumophila Dot/Icm T4SS into host cells. Our results suggest that VjbR co-ordinates expression of the T4SS and at least two of its secreted substrates.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.