ArticlePflugers Archiv : European journal of physiology2009
Aldosterone receptor sites on plasma membrane of human vascular endothelium detected by a mechanical nanosensor.
Article in Pflugers Archiv : European journal of physiology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 57 citations in OpenAlex.
- Dissecting the role of mineralocorticoid receptors in binge-like alcohol drinking in mice: Finerenone as a potential pharmacotherapy.Psychopharmacology · 2026Article
- Applying the Atomic Force Microscopy Technique in Medical Sciences-A Narrative Review.Biomedicines · 2024Review
- Aldosterone: Essential for Life but Damaging to the Vascular Endothelium.Biomolecules · 2023Review
- The Interface of Nuclear and Membrane Steroid Signaling.Endocrinology · 2021Review
- The Effects of Salt and Glucose Intake on Angiotensin II and Aldosterone in Obese and Nonobese Patients with Essential Hypertension.International journal of hypertension · 2020Article
- Endothelial Mineralocorticoid Receptor Mediates Parenchymal Arteriole and Posterior Cerebral Artery Remodeling During Angiotensin II-Induced Hypertension.Hypertension (Dallas, Tex. : 1979) · 2017Article
- Genomic and rapid effects of aldosterone: what we know and do not know thus far.Heart failure reviews · 2017Review
- Role of Nongenomic Signaling Pathways Activated by Aldosterone During Cardiac Reperfusion Injury.Molecular endocrinology (Baltimore, Md.) · 2015Article
- Salt controls endothelial and vascular phenotype.Pflugers Archiv : European journal of physiology · 2015Review
- Feedforward activation of endothelial ENaC by high sodium.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2014Article
- Aldosterone sensitizes connecting tubule glomerular feedback via the aldosterone receptor GPR30.American journal of physiology. Renal physiology · 2014Article
- The multifaceted mineralocorticoid receptor.Comprehensive Physiology · 2014Review
- The role of ENaC in vascular endothelium.Pflugers Archiv : European journal of physiology · 2014Review
- Blood pressure and amiloride-sensitive sodium channels in vascular and renal cells.Nature reviews. Nephrology · 2014Review
- Vascular endothelium leaves fingerprints on the surface of erythrocytes.Pflugers Archiv : European journal of physiology · 2013Article
- Biophysical assessment of single cell cytotoxicity: diesel exhaust particle-treated human aortic endothelial cells.PloS one · 2012Article
- Article
- Single-molecule recognition force spectroscopy of transmembrane transporters on living cells.Nature protocols · 2011Article
- Modification of the loops in the ligand-binding site turns avidin into a steroid-binding protein.BMC biotechnology · 2011Article
- Aldosterone stimulates elastogenesis in cardiac fibroblasts via mineralocorticoid receptor-independent action involving the consecutive activation of Galpha13, c-Src, the insulin-like growth factor-I receptor, and phosphatidylinositol 3-kinase/Akt.The Journal of biological chemistry · 2009Article
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The mineralocorticoid hormone aldosterone acts on target cells of kidney, colon, and the cardiovascular system through genomic and nongenomic pathways. Although the classical intracellular mineralocorticoid receptor plays a key role in mediating both pathways, it is unclear whether there are specific aldosterone receptors located on the cell surface. To search for such sites in vascular endothelium, we used an atomic force microscope (AFM) which measures unbinding forces based on single molecular recognition between an aldosterone-loaded AFM tip and the cell membrane. Aldosterone was tethered covalently via linker molecules to an AFM tip. Human endothelial cells (EA.hy926) were grown in culture and studied in buffer at 37 degrees C. Using the aldosterone-functionalized AFM tip as a mechanical nanoscale indenter, unbinding forces could be measured at randomly chosen sites of the plasma membrane. Sites with strong interactions between AFM tip and cell surface could be identified exhibiting unbinding forces of about 65 pN. The binding probability between the aldosterone-loaded tip and the cell surface at selected membrane sites was 53 +/- 7.2%. Addition of an excess supply of aldosterone to the bath solution blocked the binding of the aldosterone-loaded tip to the cell surface. The binding probability was reduced to 8.0 +/- 1.8% when an excess supply of aldosterone was added to the bath. However, it was not influenced by the addition of spironolactone or dexamethasone. We conclude that aldosterone receptor sites exist on the cell surface of vascular endothelial cells distinct from the classical mineralocorticoid receptors and insensitive to glucocorticoids. Binding of aldosterone to these receptors initiates an intracellular signaling cascade that precedes the classical genomic response and most likely participates in the control of vascular resistance.
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