ArticlePloS one2008
A green fluorescent protein with photoswitchable emission from the deep sea.
Article in PloS one, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed.
- Expression and Characterization of a Bright Far-red Fluorescent Protein from the Pink-Pigmented Tissues of Porites lobata.Marine biotechnology (New York, N.Y.) · 2020Article
- FRET-Mediated Long-Range Wavelength Transformation by Photoconvertible Fluorescent Proteins as an Efficient Mechanism to Generate Orange-Red Light in Symbiotic Deep Water Corals.International journal of molecular sciences · 2017Article
- Seeing in the deep-sea: visual adaptations in lanternfishes.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2017Review
- A novel fluorescent protein from the deep-sea anemone Cribrinopsis japonica (Anthozoa: Actiniaria).Scientific reports · 2016Article
- Fluorescent proteins function as a prey attractant: experimental evidence from the hydromedusa Olindias formosus and other marine organisms.Biology open · 2015Article
- Fluorescent protein-mediated colour polymorphism in reef corals: multicopy genes extend the adaptation/acclimatization potential to variable light environments.Molecular ecology · 2015Article
- Protein corona - from molecular adsorption to physiological complexity.Beilstein journal of nanotechnology · 2015Review
- Spectral Diversity and Regulation of Coral Fluorescence in a Mesophotic Reef Habitat in the Red Sea.PloS one · 2015Article
- The covert world of fish biofluorescence: a phylogenetically widespread and phenotypically variable phenomenon.PloS one · 2014Article
- Toward a molecular understanding of nanoparticle-protein interactions.Biophysical reviews · 2012Review
- A photoactivatable green-fluorescent protein from the phylum Ctenophora.Proceedings. Biological sciences · 2010Article
- A new bright green-emitting fluorescent protein--engineered monomeric and dimeric forms.The FEBS journal · 2010Article
Corrections and comments
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Authors and funding
9 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A colorful variety of fluorescent proteins (FPs) from marine invertebrates are utilized as genetically encoded markers for live cell imaging. The increased demand for advanced imaging techniques drives a continuous search for FPs with new and improved properties. Many useful FPs have been isolated from species adapted to sun-flooded habitats such as tropical coral reefs. It has yet remained unknown if species expressing green fluorescent protein (GFP)-like proteins also exist in the darkness of the deep sea. Using a submarine-based and -operated fluorescence detection system in the Gulf of Mexico, we discovered ceriantharians emitting bright green fluorescence in depths between 500 and 600 m and identified a GFP, named cerFP505, with bright fluorescence emission peaking at 505 nm. Spectroscopic studies showed that approximately 15% of the protein bulk feature reversible ON/OFF photoswitching that can be induced by alternating irradiation with blue und near-UV light. Despite being derived from an animal adapted to essentially complete darkness and low temperatures, cerFP505 maturation in living mammalian cells at 37 degrees C, its brightness and photostability are comparable to those of EGFP and cmFP512 from shallow water species. Therefore, our findings disclose the deep sea as a potential source of GFP-like molecular marker proteins.
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