ArticleThe Journal of biological chemistry2009
The heterogeneous nuclear ribonucleoprotein L is an essential component in the Ca2+/calmodulin-dependent protein kinase IV-regulated alternative splicing through cytidine-adenosine repeats.
Article in The Journal of biological chemistry, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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Who cites it
38 citing papers in PubMed, 56 citations in OpenAlex.
- A scaffold attachment factor PHM-2 regulates synaptic transmission through SLO-2 potassium channel in C. elegans.PLoS genetics · 2026Article
- A scaffold attachment factor PHM-2 regulates synaptic transmission through SLO-2 potassium channel inbioRxiv : the preprint server for biology · 2025Article
- Epigenetic control of adaptive or homeostatic splicing during interval-training activities.Nucleic acids research · 2024Article
- hnRNPs: roles in neurodevelopment and implication for brain disorders.Frontiers in molecular neuroscience · 2024Review
- Transcriptome-Wide Detection of Intron/Exon Definition in the Endogenous Pre-mRNA Transcripts of Mammalian Cells and Its Regulation by Depolarization.International journal of molecular sciences · 2022Article
- Multilevel Differential Control of Hormone Gene Expression Programs by hnRNP L and LL in Pituitary Cells.Molecular and cellular biology · 2018Article
- Widespread Separation of the Polypyrimidine Tract From 3' AG by G Tracts in Association With Alternative Exons in Metazoa and Plants.Frontiers in genetics · 2018Article
- Review
- Long noncoding RNA uc.345 promotes tumorigenesis of pancreatic cancer by upregulation of hnRNPL expression.Oncotarget · 2016Article
- Cutaneous tissue damage induces long-lasting nociceptive sensitization and regulation of cellular stress- and nerve injury-associated genes in sensory neurons.Experimental neurology · 2016Article
- Diverse regulation of 3' splice site usage.Cellular and molecular life sciences : CMLS · 2015Review
- Multiple effects of curcumin on promoting expression of the exon 7-containing SMN2 transcript.Genes & nutrition · 2015Article
- Evolutionary emergence of a novel splice variant with an opposite effect on the cell cycle.Molecular and cellular biology · 2015Article
- Mechanism of alternative splicing and its regulation.Biomedical reports · 2015Article
- An endogenous 'non-specific' protein detected by a His-tag antibody is human transcription regulator YY1.Data in brief · 2015Article
- Histone hyperacetylation and exon skipping: a calcium-mediated dynamic regulation in cardiomyocytes.Nucleus (Austin, Tex.) · 2015Article
- Evolutionarily emerged G tracts between the polypyrimidine tract and 3' AG are splicing silencers enriched in genes involved in cancer.BMC genomics · 2014Article
- Differential evolution of signal-responsive RNA elements and upstream factors that control alternative splicing.Cellular and molecular life sciences : CMLS · 2014Review
- Context-dependent control of alternative splicing by RNA-binding proteins.Nature reviews. Genetics · 2014Review
- Common variants in genes of the postsynaptic FMRP signalling pathway are risk factors for autism spectrum disorders.Human genetics · 2014Article
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
The regulation of gene expression through alternative pre-mRNA splicing is common in metazoans and is often controlled by intracellular signaling pathways that are important in cell physiology. We have shown that the alternative splicing of a number of genes is controlled by membrane depolarization and Ca2+/calmodulin-dependent protein kinase IV (CaMKIV) through CaMKIV-responsive RNA elements (CaRRE1 and CaRRE2); however, the trans-acting factors remain unknown. Here we show that the heterogeneous nuclear ribonucleoprotein (hnRNP) L is a CaRRE1 binding factor in nuclear extracts. An hnRNP L high affinity CA (cytidine-adenosine) repeat element is sufficient to mediate CaMKIV and hnRNP L repression of splicing in a location (3'-splice site proximity)-dependent way. Depletion of hnRNP L by RNA interference followed by rescue with coexpressed exogenous hnRNP L demonstrates that hnRNP L mediates the CaMKIV-regulated splicing through CA repeats in heterologous contexts. Depletion of hnRNP L also led to increased inclusion of the stress axis-regulated exon and a CA repeat-harboring exon under depolarization or with activated CaMKIV. Moreover, hnRNP L binding to CaRRE1 was increased by CaMKIV and, conversely, was reduced by pretreatments with protein phosphatases. Therefore, hnRNP L is an essential component of CaMKIV-regulated alternative splicing through CA repeats, with its phosphorylation likely playing a critical role.
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