ArticleGenome biology2008
Proteomics studies confirm the presence of alternative protein isoforms on a large scale.
Article in Genome biology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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Who cites it
35 citing papers in PubMed.
- Advances in Mass Spectrometry Instrumentation and Methodology for Analysis of Alternative Protein Isoforms.Journal of mass spectrometry : JMS · 2026Review
- Protein language models learn evolutionary statistics of interacting sequence motifs.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Identification of Alternative Splicing in Proteomes of Human Melanoma Cell Lines without RNA Sequencing Data.International journal of molecular sciences · 2023Article
- Origins and Evolution of Human Tandem Duplicated Exon Substitution Events.Genome biology and evolution · 2022Article
- Abnormal global alternative RNA splicing in COVID-19 patients.PLoS genetics · 2022Article
- Article
- Disordered Expression ofInternational journal of molecular sciences · 2019Article
- Alternative Splicing and Protein Diversity: Plants Versus Animals.Frontiers in plant science · 2019Review
- Unexpected conservation of the RNA splicing apparatus in the highly streamlined genome of Galdieria sulphuraria.BMC evolutionary biology · 2018Article
- Impact of Alternative Splicing on the Human Proteome.Cell reports · 2017Article
- Alternative splicing shapes transcriptome but not proteome diversity in Physcomitrella patens.Scientific reports · 2017Article
- The contribution of intrinsically disordered regions to protein function, cellular complexity, and human disease.Biochemical Society transactions · 2016Review
- Alternatively Spliced Homologous Exons Have Ancient Origins and Are Highly Expressed at the Protein Level.PLoS computational biology · 2015Article
- Article
- Comprehensive analysis of alternative splicing in Digitalis purpurea by strand-specific RNA-Seq.PloS one · 2014Article
- Review
- Discovery and mass spectrometric analysis of novel splice-junction peptides using RNA-Seq.Molecular & cellular proteomics : MCP · 2013Article
- Alternative splicing and protein interaction data sets.Nature biotechnology · 2013Article
- Function of alternative splicing.Gene · 2013Review
- Article
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundAlternative splicing of messenger RNA permits the formation of a wide range of mature RNA transcripts and has the potential to generate a diverse spectrum of functional proteins. Although there is extensive evidence for large scale alternative splicing at the transcript level, there have been no comparable studies demonstrating the existence of alternatively spliced protein isoforms.
resultsRecent advances in proteomics technology have allowed us to carry out a comprehensive identification of protein isoforms in Drosophila. The analysis of this proteomic data confirmed the presence of multiple alternative gene products for over a hundred Drosophila genes.
conclusionsWe demonstrate that proteomics techniques can detect the expression of stable alternative splice isoforms on a genome-wide scale. Many of these alternative isoforms are likely to have regions that are disordered in solution, and specific proteomics methodologies may be required to identify these peptides.
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