ArticleAntimicrobial agents and chemotherapy1991
In vitro model of attachment of Giardia intestinalis trophozoites to IEC-6 cells, an intestinal cell line.
Article in Antimicrobial agents and chemotherapy, 1991. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- Establishment of an in vitro co-infection model of Cryptosporidium parvum and Giardia duodenalis.Parasites & vectors · 2025Article
- In Vitro Models for Investigating Intestinal Host-Pathogen Interactions.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Review
- Recent advances in functional research in Giardia intestinalis.Advances in parasitology · 2020Review
- IntestinalFrontiers in physiology · 2018Review
- Angiostrongylus cantonensis cathepsin B-like protease (Ac-cathB-1) is involved in host gut penetration.Parasite (Paris, France) · 2015Article
- High-speed microscopic imaging of flagella motility and swimming in Giardia lamblia trophozoites.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- Tonic shock induces detachment of Giardia lamblia.PLoS neglected tropical diseases · 2008Article
- Giardia lamblia behavior under cytochalasins treatment.Parasitology research · 2006Article
- In vitro effects of thiazolides on Giardia lamblia WB clone C6 cultured axenically and in coculture with Caco2 cells.Antimicrobial agents and chemotherapy · 2006Article
- Inhibition of Giardia intestinalis by extracellular factors from Lactobacilli: an in vitro study.Applied and environmental microbiology · 2001Article
- Adherence of Giardia lamblia trophozoites to Int-407 human intestinal cells.Clinical and diagnostic laboratory immunology · 2001Article
- Attachment of Cryptosporidium parvum sporozoites to human intestinal epithelial cells.Infection and immunity · 1998Article
- Attachment of Toxoplasma gondii to host cells is host cell cycle dependent.Infection and immunity · 1996Article
- Growth inhibition of the intestinal parasite Giardia lamblia by a dietary lectin is associated with arrest of the cell cycle.The Journal of clinical investigation · 1994Article
- Attachment of Cryptosporidium parvum sporozoites to MDCK cells in vitro.Infection and immunity · 1994Article
- Role of surface components in the process of interaction of Giardia duodenalis with epithelial cells in vitro.Parasitology research · 1994Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Attachment of giardias to intestinal cells has been difficult to study because of a lack of a convenient in vitro model. We developed an assay for attachment of radiolabeled trophozoites to IEC-6 cells that can be done in microtiter trays. Attachment was confirmed by scanning and transmission electron microscopy. Trophozoites remained attached to the IEC-6 cells for 24 h with little evidence of damage to the IEC-6 cells. Preincubation of trophozoites with cytochalasins A, B, and D reduced attachment to approximately 20% of that of controls, whereas colchicine had no effect. Chelation of divalent cations with EDTA and EGTA [ethylene glycol-bis(beta-aminoethyl ether)-N,N,N',N'-tetraacetic acid] reduced attachment to 24 and 26% of control values, respectively, and incubation at 4 degrees C reduced attachment to 7% of the value for controls incubated at 37 degrees C. Glutaraldehyde fixation of trophozoites or IEC-6 cells resulted in significantly diminished attachment to the live substrate (17 and 40% of control values, respectively). Coincubation of IEC-6 cells and trophozoites on a rotary shaker resulted in detachment of 40% of trophozoites, but EDTA, EGTA, glutaraldehyde fixation of trophozoites, and low temperature diminished attachment markedly and significantly. Similar results were obtained in selected experiments with three strains of giardia.
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