ReviewFEMS microbiology reviews2009
Engineering prokaryotic gene circuits.
Review in FEMS microbiology reviews, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
21 citing papers in PubMed, 45 citations in OpenAlex.
- Optimized cumate toolkit for tunable protein expression duringApplied and environmental microbiology · 2026Article
- A comprehensive review of genomic-scale genetic engineering as a strategy to improve bacterial productivity.Microbiology (Reading, England) · 2025Review
- Engineering bacterial theranostics: from logic gates toFrontiers in bioengineering and biotechnology · 2024Review
- Principles of gene regulation quantitatively connect DNA to RNA and proteins in bacteria.Science (New York, N.Y.) · 2022Article
- Design of typical genes for heterologous gene expression.Scientific reports · 2022Article
- Engineered systems of inducible anti-repressors for the next generation of biological programming.Nature communications · 2020Article
- Holistic engineering of cell-free systems through proteome-reprogramming synthetic circuits.Nature communications · 2020Article
- Transcriptional programming using engineered systems of transcription factors and genetic architectures.Nature communications · 2019Article
- Recombinant Heparin-New Opportunities.Frontiers in medicine · 2018Review
- Engineering microbial phenotypes through rewiring of genetic networks.Nucleic acids research · 2017Article
- Engineering orthogonal dual transcription factors for multi-input synthetic promoters.Nature communications · 2016Article
- A genetic biosensor for identification of transcriptional repressors of target promoters.Scientific reports · 2015Article
- Nutritional Control of DNA Replication Initiation through the Proteolysis and Regulated Translation of DnaA.PLoS genetics · 2015Article
- Opportunities for synthetic biology in antibiotics: expanding glycopeptide chemical diversity.ACS synthetic biology · 2015Review
- Comprehensive analysis of the Corynebacterium glutamicum transcriptome using an improved RNAseq technique.BMC genomics · 2013Article
- The Standard European Vector Architecture (SEVA): a coherent platform for the analysis and deployment of complex prokaryotic phenotypes.Nucleic acids research · 2013Article
- Metabolic engineering of Chinese hamster ovary cells: towards a bioengineered heparin.Metabolic engineering · 2012Article
- Novel in vivo-degradable cellulose-chitin copolymer from metabolically engineered Gluconacetobacter xylinus.Applied and environmental microbiology · 2010Article
- Synthetic biology: tools to design, build, and optimize cellular processes.Journal of biomedicine & biotechnology · 2010Review
- You're one in a googol: optimizing genes for protein expression.Journal of the Royal Society, Interface · 2009Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Engineering of synthetic gene circuits is a rapidly growing discipline, currently dominated by prokaryotic transcription networks, which can be easily rearranged or rewired to give different output behaviours. In this review, we examine both a rational and a combinatorial design of such networks and discuss progress on using in vitro evolution techniques to obtain functional systems. Moving beyond pure transcription networks, more and more networks are being implemented at the level of RNA, taking advantage of mechanisms of translational control and aptamer-small molecule complex formation. Unlike gene expression systems, metabolic components are generally not as interconnectable in any combination, and so engineering of metabolic circuits is a particularly challenging field. Nonetheless, metabolic engineering has immense potential to provide useful biosynthesis tools for biotechnology applications. Finally, although prokaryotes are mostly studied as single cell systems, cell-cell communication networks are now being developed that result in spatial pattern formation in multicellular prokaryote colonies. This represents a crossover with multicellular organisms, showing that prokaryotic systems have the potential to tackle questions traditionally associated with developmental biology. Overall, the current advances in synthetic gene synthesis, ultra-high-throughput DNA sequencing and computation are synergizing to drive synthetic gene network design at an unprecedented pace.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.