ArticleChromosoma2009
Artificial chromosome formation in maize (Zea mays L.).
Article in Chromosoma, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
30 citing papers in PubMed, 84 citations in OpenAlex.
- Unambiguous chromosome identification by multicolour fluorescence in situ hybridisation reveals the genome organisation and evolution of sheepgrass (Leymus chinensis).BMC plant biology · 2026Article
- History and current status of embryogenic culture-based tissue culture, transformation and gene editing of maize (Zea mays L.).The plant genome · 2025Review
- A key to totipotency: Wuschel-like homeobox 2a unlocks embryogenic culture response in maize (Zea mays L.).Plant biotechnology journal · 2023Article
- Artificial chromosome technology and its potential application in plants.Frontiers in plant science · 2022Review
- Biocircuits in plants and eukaryotic algae.Frontiers in plant science · 2022Review
- Maize transformation: history, progress, and perspectives.Molecular breeding : new strategies in plant improvement · 2021Review
- CRISPR-Cas9 Editing in Maize: Systematic Evaluation of Off-target Activity and Its Relevance in Crop Improvement.Scientific reports · 2019Article
- Using Morphogenic Genes to Improve Recovery and Regeneration of Transgenic Plants.Plants (Basel, Switzerland) · 2019Review
- Molecular identification of the wheat male fertility gene Ms1 and its prospects for hybrid breeding.Nature communications · 2017Article
- Targeted mutagenesis of a conserved anther-expressed P450 gene confers male sterility in monocots.Plant biotechnology journal · 2017Article
- ARGOS8 variants generated by CRISPR-Cas9 improve maize grain yield under field drought stress conditions.Plant biotechnology journal · 2017Article
- Genome editing in maize directed by CRISPR-Cas9 ribonucleoprotein complexes.Nature communications · 2016Article
- Morphogenic RegulatorsThe Plant cell · 2016Article
- Plant artificial chromosome technology and its potential application in genetic engineering.Plant biotechnology journal · 2016Review
- Rapid characterization of CRISPR-Cas9 protospacer adjacent motif sequence elements.Genome biology · 2015Article
- Targeted Mutagenesis, Precise Gene Editing, and Site-Specific Gene Insertion in Maize Using Cas9 and Guide RNA.Plant physiology · 2015Article
- FISH and GISH: molecular cytogenetic tools and their applications in ornamental plants.Plant cell reports · 2015Article
- Engineering of plant chromosomes.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2015Review
- Circular permutation of a synthetic eukaryotic chromosome with the telomerator.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Minichromosomes and artificial chromosomes in Arabidopsis.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2014Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We report on the construction of maize minichromosomes using shuttle vectors harboring native centromeric segments, origins of replication, selectable marker genes, and telomeric repeats. These vectors were introduced into scutellar cells of maize immature embryos by microprojectile bombardment. Several independent transformation events were identified containing minichromosomes in addition to the normal diploid complement of 20 maize chromosomes. Immunostaining indicated that the minichromosomes recruited centromeric protein C, which is a specific component of the centromere/kinetochore complex. Minichromosomes were estimated to be 15-30 Mb in size based on cytological measurements. Fluorescent in situ hybridization (FISH) showed that minichromosomes contain the centromeric, telomeric, and exogenous unique marker sequences interspersed with maize retrotransposons. Minichromosomes were detected for at least a year in actively dividing callus cultures, providing evidence for their stability through numerous cell cycles. Plants were regenerated and minichromosomes were detected in root tips, providing confirmation of their normal replication and transmission during mitosis and through organogenesis. Assembly of maize artificial chromosomes may provide a tool to study centromere function and a foundation for developing new high capacity vectors for plant functional genomics and breeding.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.