ArticlePlant cell reports2009
Protamine-mediated DNA coating remarkably improves bombardment transformation efficiency in plant cells.
Article in Plant cell reports, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 63 citations in OpenAlex.
- Powdery mildew effectors AVRMolecular plant pathology · 2024Article
- Particle bombardment technology and its applications in plants.Molecular biology reports · 2020Review
- Auxin apical dominance governed by the OsAsp1-OsTIF1 complex determines distinctive rice caryopses development on different branches.PLoS genetics · 2020Article
- Protamine-stabilized gold nanoclusters as a fluorescent nanoprobe for lead(II) via Pb(II)-Au(I) interaction.Mikrochimica acta · 2018Article
- Particle bombardment - mediated gene transfer and GFP transient expression in Seteria viridis.Plant signaling & behavior · 2018Article
- The effect of chitosan-PMAA-NPK nanofertilizer on3 Biotech · 2018Article
- Advancing Crop Transformation in the Era of Genome Editing.The Plant cell · 2016Review
- Production of functional native human interleukin-2 in tobacco chloroplasts.Molecular biotechnology · 2014Article
- Maize transformation technology development for commercial event generation.Frontiers in plant science · 2014Review
- Blast resistance of CC-NB-LRR protein Pb1 is mediated by WRKY45 through protein-protein interaction.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- The LuWD40-1 gene encoding WD repeat protein regulates growth and pollen viability in flax (Linum Usitatissimum L.).PloS one · 2013Article
- Association of poly I:C RNA and plasmid DNA onto MnO nanorods mediated by PAMAM.Langmuir : the ACS journal of surfaces and colloids · 2012Article
- Switchgrass (Panicum virgatum L.) polyubiquitin gene (PvUbi1 and PvUbi2) promoters for use in plant transformation.BMC biotechnology · 2011Article
- Generation of transgenic C. elegans by biolistic transformation.Journal of visualized experiments : JoVE · 2010Article
- Characterization and performance of nucleic acid nanoparticles combined with protamine and gold.Biomaterials · 2009Article
Corrections and comments
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Authors and funding
3 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have developed a method by which remarkably higher efficiencies of transient and stable transformation were achieved in bombardment transformation of plants. Over fivefold increase in transient gus gene expression was achieved when rice or maize suspension cells were bombarded with gold particles coated with plasmid DNA in the presence of protamine instead of the conventional spermidine. A 3.3-fold improvement in stable transformation efficiency was also observed using rice suspension cells with the new coating approach. The coated protamine-plasmid DNA complex resisted degradation by a DNase or by rice cell extract much longer than the spermidine-plasmid DNA complex. The results from this study suggest that protamine protects plasmid DNA longer than spermidine when being delivered inside the cells, probably by forming a nano-scale complex, and thus helps improve the efficiency of particle bombardment-mediated plant transformation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.