ArticleEuropean biophysics journal : EBJ2009
Probing complexes with single fluorophores: factors contributing to dispersion of FRET in DNA/RNA duplexes.
Article in European biophysics journal : EBJ, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed.
- 3D maps of RNA interhelical junctions.Nature protocols · 2011Article
- Three-dimensional molecular modeling with single molecule FRET.Journal of structural biology · 2011Review
- The emerging field of RNA nanotechnology.Nature nanotechnology · 2010Review
- Dual-channel single-molecule fluorescence resonance energy transfer to establish distance parameters for RNA nanoparticles.ACS nano · 2010Article
- Optimizing methods to recover absolute FRET efficiency from immobilized single molecules.Biophysical journal · 2010Article
- Stoichiometry of a regulatory splicing complex revealed by single-molecule analyses.The EMBO journal · 2010Article
- Fluorescence lifetime measurements and biological imaging.Chemical reviews · 2010Review
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
Single molecule fluorescent microscopy is a method for the analysis of the dynamics of biological macromolecules by detecting the fluorescence signal produced by fluorophores associated with the macromolecule. Two fluorophores located in a close proximity may result in Förster resonance energy transfer (FRET), which can be detected at the single molecule level and the efficiency of energy transfer calculated. In most cases, the experimentally observed distribution of FRET efficiency exhibits a significant width corresponding to 0.07-0.2 (on a scale of 0-1). Here, we present a general approach describing the analysis of experimental data for a DNA/RNA duplex. We have found that for a 15 bp duplex with Cy3 and Cy5 fluorophores attached to the opposite ends of the helix, the width of the energy transfer distribution is mainly determined by the photon shot noise and the orientation factor, whereas the variation of inter-dye distances plays a minor role.
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Registered trials
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