ArticlePLoS neglected tropical diseases2008
Quantitative detection of Schistosoma japonicum cercariae in water by real-time PCR.
Article in PLoS neglected tropical diseases, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers.
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Who cites it
44 citing papers in PubMed, 71 citations in OpenAlex.
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- Mammalian and Avian Larval Schistosomatids in Bangladesh: Molecular Characterization, Epidemiology, Molluscan Vectors, and Occurrence of Human Cercarial Dermatitis.Pathogens (Basel, Switzerland) · 2022Article
- Specific Nucleic AcId Ligation for the detection of Schistosomes: SNAILS.PLoS neglected tropical diseases · 2022Article
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- Development of a novel real-time polymerase chain reaction assay for the sensitive detection of Schistosoma japonicum in human stool.PLoS neglected tropical diseases · 2021Article
- Low Sensitivity of Real Time PCRs Targeting Retrotransposon Sequences for the Detection ofPathogens (Basel, Switzerland) · 2021Article
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- Environmental DNA-based xenomonitoring for determining Schistosoma presence in tropical freshwaters.Parasites & vectors · 2020Article
- Analysis of Environmental DNA and Edaphic Factors for the Detection of the Snail Intermediate HostPathogens (Basel, Switzerland) · 2019Article
- Environmental DNA for improved detection and environmental surveillance of schistosomiasis.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Odds, challenges and new approaches in the control of helminthiasis, an Asian study.Parasite epidemiology and control · 2019Review
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- DNA Diagnostics for Schistosomiasis Control.Tropical medicine and infectious disease · 2018Review
Corrections and comments
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Authors and funding
2 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In China alone, an estimated 30 million people are at risk of schistosomiasis, caused by the Schistosoma japonicum parasite. Disease has re-emerged in several regions that had previously attained transmission control, reinforcing the need for active surveillance. The environmental stage of the parasite is known to exhibit high spatial and temporal variability, and current detection techniques rely on a sentinel mouse method which has serious limitations in obtaining data in both time and space. Here we describe a real-time PCR assay to quantitatively detect S. japonicum cercariae in laboratory samples and in natural water that has been spiked with known numbers of S. japonicum. Multiple primers were designed and assessed, and the best performing set, along with a TaqMan probe, was used to quantify S. japonicum. The resulting assay was selective, with no amplification detected for Schistosoma mansoni, Schistosoma haematobium, avian schistosomes nor organisms present in non-endemic surface water samples. Repeated samples containing various concentrations of S. japonicum cercariae showed that the real-time PCR method had a strong linear correlation (R(2) = 0.921) with light microscopy counts, and the detection limit was below the DNA equivalent of half of one cercaria. Various cercarial concentrations spiked in 1 liter of natural water followed by a filtration process produced positive detection from 93% of samples analyzed. The real-time PCR method performed well quantifying the relative concentrations of various spiked samples, although the absolute concentration estimates exhibited high variance across replicated samples. Overall, the method has the potential to be applied to environmental water samples to produce a rapid, reliable assay for cercarial location in endemic areas.
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