Evidence map›Paper›PMID 19015548›Full record

ArticleGenetics2009

A high-density single nucleotide polymorphism map for Neurospora crassa.

Randy Lambreghts, Mi Shi, William J Belden, David Decaprio, Danny Park, Matthew R Henn, James E Galagan, Meray Bastürkmen, Bruce W Birren, Matthew S Sachs and 2 more

Abstract readValidation Study
In one paragraph

Article in Genetics, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.

0numbers the graph read from it
0cells of the map it votes in
31citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

31 citing papers in PubMed.

  1. Article
  2. Outstanding women scientists who have broadened the knowledge on biological photoreceptors-II.Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology · 2024
    Article
  3. Article
  4. Article
  5. Article
  6. Article
  7. Article
  8. Article
  9. The circadian system as an organizer of metabolism.Fungal genetics and biology : FG & B · 2016
    Review
  10. Review
  11. Review
  12. Article
  13. Article
  14. Article
  15. Article
  16. Article
  17. Article
  18. The circadian clock of Neurospora crassa.FEMS microbiology reviews · 2012
    Review
  19. Article
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Randy LambreghtsDepartment of Environmental and Biomolecular Systems, Oregon Health & Science University, Beaverton, Oregon 97006, USA.
Mi Shi
William J Belden
David Decaprio
Danny Park
Matthew R Henn
James E Galagan
Meray Bastürkmen
Bruce W Birren
Matthew S Sachs
Jay C Dunlap
Jennifer J Loros

Funding

Tools for Functional Analysis and Cell BiologyP01GM068087 · NIGMS · DARTMOUTH COLLEGE · PI GALAGAN, JAMES E · 2004 to 2013
$19.3M
Identification and Analysis of Circadian Clock-Controlled GenesR01GM083336 · NIGMS · DARTMOUTH COLLEGE · PI LOROS, JENNIFER J. · 2008 to 2015
$5.7M
GENETIC AND MOLECULAR DISSECTION OF THE NEUROSPORA CLOCKR01GM034985 · NIGMS · DARTMOUTH COLLEGE · PI DUNLAP, JAY C. · 1986 to 2015
$5.7M
GENETIC AND MOLECULAR DISSECTION OF THE NEUSPORA CLOCKR37GM034985 · NIGMS · DARTMOUTH COLLEGE · PI DUNLAP, JAY C · 1998 to 2007
$4.4M
Role of Chromatin Remodeling in Circadian RegulationF32GM071223 · NIGMS · DARTMOUTH COLLEGE · PI BELDEN, WILLIAM J · 2004 to 2006
$149k
NIGMS NIH HHS F32 GM071223NIGMS NIH HHS GM034985NIGMS NIH HHS GM068087NIGMS NIH HHS GM071223NIGMS NIH HHS GM083336NIGMS NIH HHS P01 GM068087NIGMS NIH HHS R01 GM034985NIGMS NIH HHS R01 GM083336NIGMS NIH HHS R37 GM034985
6 · The paper itself

Abstract

We report the discovery and validation of a set of single nucleotide polymorphisms (SNPs) between the reference Neurospora crassa strain Oak Ridge and the Mauriceville strain (FGSC 2555), of sufficient density to allow fine mapping of most loci. Sequencing of Mauriceville cDNAs and alignment to the completed genomic sequence of the Oak Ridge strain identified 19,087 putative SNPs. Of these, a subset was validated by cleaved amplified polymorphic sequence (CAPS), a simple and robust PCR-based assay that reliably distinguishes between SNP alleles. Experimental confirmation resulted in the development of 250 CAPS markers distributed evenly over the genome. To demonstrate the applicability of this map, we used bulked segregant analysis followed by interval mapping to locate the csp-1 mutation to a narrow region on LGI. Subsequently, we refined mapping resolution to 74 kbp by developing additional markers, resequenced the candidate gene, NCU02713.3, in the mutant background, and phenocopied the mutation by gene replacement in the WT strain. Together, these techniques demonstrate a generally applicable and straightforward approach for the isolation of novel genes from existing mutants. Data on both putative and validated SNPs are deposited in a customized public database at the Broad Institute, which encourages augmentation by community users.

Indexed as

Polymorphism, Single NucleotideChromosome MappingDatabases, Nucleic AcidDNA, FungalExpressed Sequence TagsGenes, FungalGenetic MarkersMutationNeurospora crassaPolymerase Chain ReactionRecombination, GeneticSpecies SpecificityDNA, FungalGenetic Markers

Identifiers

PMID19015548
PMCPMC2644964

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.