ArticleCirculation2008
Electrophysiological consequences of acute regional ischemia/reperfusion in neonatal rat ventricular myocyte monolayers.
Article in Circulation, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed.
- Differential response to acute ischemia between isolated contracting hearts and hearts perfused with excitation-contraction uncouplers.American journal of physiology. Heart and circulatory physiology · 2026Article
- β2-Adrenergic Receptor Agonist Clenbuterol Protects Against Acute Ischemia/Reperfusion-Induced Arrhythmia by Regulation of Akt/eNOS/NO/Cx43 Signaling Pathway.Pharmacology research & perspectives · 2025Article
- Article
- Bridging the Translational Gap in Heart Failure Research: Using Human iPSC-derived Cardiomyocytes to Accelerate Therapeutic Insights.Methodist DeBakey cardiovascular journal · 2023Review
- Serine-threonine protein phosphatase regulation of Cx43 dephosphorylation in arrhythmogenic disorders.Cellular signalling · 2021Review
- Arrhythmogenic risks of stem cell replacement therapy for cardiovascular diseases.Journal of cellular physiology · 2020Review
- Connexin 43 dephosphorylation contributes to arrhythmias and cardiomyocyte apoptosis in ischemia/reperfusion hearts.Basic research in cardiology · 2019Article
- Pro-arrhythmic Effects of Hydrogen Sulfide in Healthy and Ischemic Cardiac Tissues: Insight From a Simulation Study.Frontiers in physiology · 2019Article
- Sevoflurane Alleviates Reperfusion Arrhythmia by Ameliorating TDR and MAPDAnesthesiology research and practice · 2019Article
- Article
- Icariin, a Novel Blocker of Sodium and Calcium Channels, Eliminates Early and Delayed Afterdepolarizations, As Well As Triggered Activity, in Rabbit Cardiomyocytes.Frontiers in physiology · 2017Article
- Engineering prokaryotic channels for control of mammalian tissue excitability.Nature communications · 2016Article
- Islands of spatially discordant APD alternans underlie arrhythmogenesis by promoting electrotonic dyssynchrony in models of fibrotic rat ventricular myocardium.Scientific reports · 2016Article
- Mitochondrial instability during regional ischemia-reperfusion underlies arrhythmias in monolayers of cardiomyocytes.Journal of molecular and cellular cardiology · 2015Article
- Stem cells can form gap junctions with cardiac myocytes and exert pro-arrhythmic effects.Frontiers in physiology · 2014Review
- Ratiometric imaging of calcium during ischemia-reperfusion injury in isolated mouse hearts using Fura-2.Biomedical engineering online · 2012Article
- Nutrient restriction preserves calcium cycling and mitochondrial function in cardiac myocytes during ischemia and reperfusion.Cell calcium · 2012Article
- Regional increase in extracellular potassium can be arrhythmogenic due to nonuniform muscle contraction in rat ventricular muscle.American journal of physiology. Heart and circulatory physiology · 2012Article
- Single-detector simultaneous optical mapping of V(m) and [Ca(2+)](i) in cardiac monolayers.Annals of biomedical engineering · 2012Article
- Optical imaging of voltage and calcium in cardiac cells & tissues.Circulation research · 2012Review
Corrections and comments
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Authors and funding
7 authors.
Funding
Abstract
backgroundElectrophysiological changes promoting arrhythmias during acute regional ischemia/reperfusion are challenging to study in intact cardiac tissue because of complex 3-dimensional myocardial and vascular geometry. We characterized electrophysiological alterations and arrhythmias during regional ischemia/reperfusion in a simpler 2-dimensional geometry of cultured neonatal rat ventricular myocyte monolayers. METHODS AND
resultsOptical mapping of intracellular Ca (Ca(i)) and voltage was performed with the use of Rhod 2-AM and Rh-237, respectively. Regional ischemia was mimicked by covering the central portion of monolayer with a glass coverslip, and reperfusion was mimicked by removing the coverslip. Monolayers were stained with fluorescent antibodies to detect total and dephosphorylated connexin-43 at various time points. During coverslip ischemia, action potential duration shortened, Ca(i) transient duration was prolonged, and local conduction velocity (CV) slowed progressively, with loss of excitability after 10.6 +/- 3.6 minutes. CV slowing was accompanied by connexin-43 dephosphorylation. During ischemia, spontaneous reentry occurred in 5 of 11 monolayers, initiated by extrasystoles arising from the border zone or unidirectional conduction block of paced beats. On reperfusion, excitability recovered within 1.0 +/- 0.8 minutes, but CV remained depressed for 9.0 +/- 3.0 minutes, promoting reentry in the reperfused zone. As connexin-43 phosphorylation recovered in the reperfused zone, CV normalized, and arrhythmias resolved.
conclusionsAcute regional ischemia/reperfusion in neonatal rat ventricular myocyte monolayers recapitulates electrophysiological alterations and arrhythmias similar to those observed during acute coronary occlusion/reperfusion in intact hearts. During early reperfusion, slow recovery from connexin-43 dephosphorylation leads to persistent CV slowing, creating a highly arrhythmogenic substrate.
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Registered trials
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