ArticleMolecular and cellular biology2009
CUX1 and E2F1 regulate coordinated expression of the mitotic complex genes Ect2, MgcRacGAP, and MKLP1 in S phase.
Article in Molecular and cellular biology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed, 46 citations in OpenAlex.
- KIF23 in disease pathogenesis and therapeutics : from molecular mechanisms to clinical translation.Cancer cell international · 2026Review
- Contribution of ECT2 to Tubulointerstitial Fibrosis in the Progression of Chronic Kidney Disease.Current medical science · 2024Article
- Identification and characterization of the mediator kinase-dependent myometrial stem cell phosphoproteome.F&S science · 2021Article
- Article
- Epithelial cell transforming 2 is regulated by Yes-associated protein 1 and mediates pancreatic cancer progression and metastasis.American journal of physiology. Gastrointestinal and liver physiology · 2021Article
- KIF23 enhances cell proliferation in pancreatic ductal adenocarcinoma and is a potent therapeutic target.Annals of translational medicine · 2020Article
- Integrative analyses of gene expression profile reveal potential crucial roles of mitotic cell cycle and microtubule cytoskeleton in pulmonary artery hypertension.BMC medical genomics · 2020Article
- Integrated systems-genetic analyses reveal a network target for delaying glioma progression.Annals of clinical and translational neurology · 2019Article
- Polymerase-δ-interacting protein 2 activates the RhoGEF epithelial cell transforming sequence 2 in vascular smooth muscle cells.American journal of physiology. Cell physiology · 2019Article
- A 'synthetic-sickness' screen for senescence re-engagement targets in mutant cancer backgrounds.PLoS genetics · 2017Article
- Mutation analysis and copy number alterations ofOncoTargets and therapy · 2017Article
- MicroRNA-122 regulates polyploidization in the murine liver.Hepatology (Baltimore, Md.) · 2016Article
- Prognostic significance of FOXM1 expression and antitumor effect of FOXM1 inhibition in synovial sarcomas.BMC cancer · 2016Article
- The dynamic behavior of Ect2 in response to DNA damage.Scientific reports · 2016Article
- Transcriptional control of mitosis: deregulation and cancer.Frontiers in endocrinology · 2015Review
- Identification of a mitotic Rac-GEF, Trio, that counteracts MgcRacGAP function during cytokinesis.Molecular biology of the cell · 2014Article
- CUX1, a haploinsufficient tumour suppressor gene overexpressed in advanced cancers.Nature reviews. Cancer · 2014Review
- Suppressor of cytokine signaling 1 modulates invasion and metastatic potential of colorectal cancer cells.Molecular oncology · 2014Article
- Rho GTPases as regulators of mitosis and cytokinesis in mammalian cells.Small GTPases · 2014Review
- Long-range transcriptional regulation by the p110 CUX1 homeodomain protein on the ENCODE array.BMC genomics · 2013Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Rho GTPases are critical for mitosis progression and completion of cytokinesis. During mitosis, the GDP/GTP cycle of Rho GTPases is regulated by the exchange factor Ect2 and the GTPase activating protein MgcRacGAP which associates with the kinesin MKLP1 in the centralspindlin complex. We report here that expression of Ect2, MgcRacGAP, and MKLP1 is tightly regulated during cell cycle progression. These three genes share similar cell cycle-related signatures within their promoter regions: (i) cell cycle gene homology region (CHR) sites located at -20 to +40 nucleotides of their transcription start sites that are required for repression in G(1), (ii) E2F binding elements, and (iii) tandem repeats of target sequences for the CUX1 transcription factor. CUX1 and E2F1 bind these three promoters upon S-phase entry, as demonstrated by chromatin immunoprecipitation, and regulate transcription of these genes, as established using promoter-luciferase reporter constructs and expression of activated or dominant negative transcription factors. Overexpression of either E2F1 or CUX1 increased the levels of the endogenous proteins whereas small interfering RNA knockdown of E2F1 or use of a dominant negative E2F1 reduced their expression levels. Thus, CUX1, E2F, and CHR elements provide the transcriptional controls that coordinate induction of Ect2, MgcRacGAP, and MKLP1 in S phase, leading to peak expression of these interacting proteins in G(2)/M, at the time they are required to regulate cytokinesis.
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