ArticleArchives of biochemistry and biophysics2009
Ethanol exposure decreases mitochondrial outer membrane permeability in cultured rat hepatocytes.
Article in Archives of biochemistry and biophysics, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
25 citing papers in PubMed, 58 citations in OpenAlex.
- ONC201-Induced Mitochondrial Dysfunction, Senescence-like Phenotype, and Sensitization of Cultured BT474 Human Breast Cancer Cells to TRAIL.International journal of molecular sciences · 2022Article
- Age-Related Features of the Viscosity of Plasma and Mitochondrial Membranes of Hepatocytes in Liver Cirrhosis.Bulletin of experimental biology and medicine · 2021Article
- Metabolic implications of non-electrogenic ATP/ADP exchange in cancer cells: A mechanistic basis for the Warburg effect.Biochimica et biophysica acta. Bioenergetics · 2021Review
- The gut microbiome and its potential role in paradoxical anaerobism in pupfishes of the Mojave Desert.Animal microbiome · 2020Article
- Alcohol effects on hepatic lipid metabolism.Journal of lipid research · 2020Review
- Redox-dependent gating of VDAC by mitoNEET.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Role of mitochondrial depolarization and disrupted mitochondrial homeostasis in non-alcoholic steatohepatitis and fibrosis in mice.International journal of physiology, pathophysiology and pharmacology · 2019Article
- A Unifying Hypothesis Linking Hepatic Adaptations for Ethanol Metabolism to the Proinflammatory and Profibrotic Events of Alcoholic Liver Disease.Alcoholism, clinical and experimental research · 2018Review
- Mitophagy in hepatocytes: Types, initiators and role in adaptive ethanol metabolism☆.Liver research (Beijing, China) · 2018Article
- Myrtle berries seeds aqueous extract abrogates chronic alcohol consumption-induced erythrocytes osmotic stability disturbance, haematological and biochemical toxicity.Lipids in health and disease · 2018Article
- VDAC Regulation: A Mitochondrial Target to Stop Cell Proliferation.Advances in cancer research · 2018Review
- VDAC-Tubulin, an Anti-Warburg Pro-Oxidant Switch.Frontiers in oncology · 2017Review
- Evolution of Voltage-Dependent Anion Channel Function: From Molecular Sieve to Governator to Actuator of Ferroptosis.Frontiers in oncology · 2017Article
- TGF-β1-mediated differentiation of fibroblasts is associated with increased mitochondrial content and cellular respiration.PloS one · 2015Article
- Acute ethanol causes hepatic mitochondrial depolarization in mice: role of ethanol metabolism.PloS one · 2014Article
- Regulation of mitochondrial function by voltage dependent anion channels in ethanol metabolism and the Warburg effect.Biochimica et biophysica acta · 2012Review
- VDAC inhibition by tubulin and its physiological implications.Biochimica et biophysica acta · 2012Review
- Chi-square analysis of the reduction of ATP levels in L-02 hepatocytes by hexavalent chromium.Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologica · 2012Article
- Ethanol suppresses ureagenesis in rat hepatocytes: role of acetaldehyde.The Journal of biological chemistry · 2012Article
- VDAC1: from structure to cancer therapy.Frontiers in oncology · 2012Article
Corrections and comments
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Authors and funding
2 authors at 2 institutions in 2 countries.
Funding
Abstract
Mitochondrial metabolism depends on movement of hydrophilic metabolites through the mitochondrial outer membrane via the voltage-dependent anion channel (VDAC). Here we assessed VDAC permeability of intracellular mitochondria in cultured hepatocytes after plasma membrane permeabilization with 8 microM digitonin. Blockade of VDAC with Koenig's polyanion inhibited uncoupled and ADP-stimulated respiration of permeabilized hepatocytes by 33% and 41%, respectively. Tenfold greater digitonin (80 microM) relieved KPA-induced inhibition and also released cytochrome c, signifying mitochondrial outer membrane permeabilization. Acute ethanol exposure also decreased respiration and accessibility of mitochondrial adenylate kinase (AK) of permeabilized hepatocytes membranes by 40% and 32%, respectively. This inhibition was reversed by high digitonin. Outer membrane permeability was independently assessed by confocal microscopy from entrapment of 3 kDa tetramethylrhodamine-conjugated dextran (RhoDex) in mitochondria of mechanically permeabilized hepatocytes. Ethanol decreased RhoDex entrapment in mitochondria by 35% of that observed in control cells. Overall, these results demonstrate that acute ethanol exposure decreases mitochondrial outer membrane permeability most likely by inhibition of VDAC.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.