ArticleBMC molecular biology2008
Identification of importin 8 (IPO8) as the most accurate reference gene for the clinicopathological analysis of lung specimens.
Article in BMC molecular biology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers, 2 of them syntheses that pooled it.
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Who cites it
30 citing papers in PubMed, 2 syntheses or guidelines pooled it.
- Varied pathways of stage IA lung adenocarcinomas discovered by integrated gene expression analysis.International journal of biological sciences · 2011Pooled it
- Identification of reference genes across physiological states for qRT-PCR through microarray meta-analysis.PloS one · 2011Pooled it
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- Identification of new reference genes with stable expression patterns for cell cycle experiments in human leukemia cell lines.Scientific reports · 2025Article
- Novel genome-wide DNA methylation profiling reveals distinct epigenetic landscape, prognostic model and cellular composition of early-stage lung adenocarcinoma.Journal of translational medicine · 2024Article
- Construction and validation of a prognostic model for stemness-related genes in lung adenocarcinoma.Translational cancer research · 2024Article
- Epigenetic Alterations of DNA Methylation and miRNA Contribution to Lung Adenocarcinoma.Frontiers in genetics · 2022Article
- RNA Sequencing of Tumor-Educated Platelets Reveals a Three-Gene Diagnostic Signature in Esophageal Squamous Cell Carcinoma.Frontiers in oncology · 2022Article
- Complex Alterations of Fatty Acid Metabolism and Phospholipidome Uncovered in Isolated Colon Cancer Epithelial Cells.International journal of molecular sciences · 2021Article
- The remodelling of actin composition as a hallmark of cancer.Translational oncology · 2021Review
- Pediatric Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2): Clinical Presentation, Infectivity, and Immune Responses.The Journal of pediatrics · 2020Article
- Peripheral blood lymphocyte proviral DNA predicts neurocognitive impairment in clade C HIV.Journal of neurovirology · 2020Article
- Reactive Oxygen Species, Metabolic Plasticity, and Drug Resistance in Cancer.International journal of molecular sciences · 2020Review
- Evaluation of suitable reference genes for normalization of quantitative reverse transcription PCR analyses in Clavibacter michiganensis.MicrobiologyOpen · 2019Article
- miRNA processing gene polymorphisms, blood DNA methylation age and long-term ambient PMEpigenomics · 2017Article
- Reference gene validation for gene expression normalization in canine osteosarcoma: a geNorm algorithm approach.BMC veterinary research · 2017Article
- A large-scale analysis of alternative splicing reveals a key role of QKI in lung cancer.Molecular oncology · 2016Article
- Expression of sirtuin 1 and 2 is associated with poor prognosis in non-small cell lung cancer patients.PloS one · 2015Article
- Long noncoding RNA HOTAIR is relevant to cellular proliferation, invasiveness, and clinical relapse in small-cell lung cancer.Cancer medicine · 2014Article
- Systems biology approach to stage-wise characterization of epigenetic genes in lung adenocarcinoma.BMC systems biology · 2013Article
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Authors and funding
12 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe accurate normalization of differentially expressed genes in lung cancer is essential for the identification of novel therapeutic targets and biomarkers by real time RT-PCR and microarrays. Although classical "housekeeping" genes, such as GAPDH, HPRT1, and beta-actin have been widely used in the past, their accuracy as reference genes for lung tissues has not been proven.
resultsWe have conducted a thorough analysis of a panel of 16 candidate reference genes for lung specimens and lung cell lines. Gene expression was measured by quantitative real time RT-PCR and expression stability was analyzed with the softwares GeNorm and NormFinder, mean of |Delta Ct| (= |Ct Normal-Ct tumor|) +/- SEM, and correlation coefficients among genes. Systematic comparison between candidates led us to the identification of a subset of suitable reference genes for clinical samples: IPO8, ACTB, POLR2A, 18S, and PPIA. Further analysis showed that IPO8 had a very low mean of |Delta Ct| (0.70 +/- 0.09), with no statistically significant differences between normal and malignant samples and with excellent expression stability.
conclusionOur data show that IPO8 is the most accurate reference gene for clinical lung specimens. In addition, we demonstrate that the commonly used genes GAPDH and HPRT1 are inappropriate to normalize data derived from lung biopsies, although they are suitable as reference genes for lung cell lines. We thus propose IPO8 as a novel reference gene for lung cancer samples.
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