ArticleBMC systems biology2008
Stochastic analysis of the GAL genetic switch in Saccharomyces cerevisiae: modeling and experiments reveal hierarchy in glucose repression.
Article in BMC systems biology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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Who cites it
4 citing papers in PubMed.
- Noise reduction facilitated by dosage compensation in gene networks.Nature communications · 2016Article
- A cell size- and cell cycle-aware stochastic model for predicting time-dynamic gene network activity in individual cells.BMC systems biology · 2015Article
- Application of structure equation modeling for inferring a serial transcriptional regulation in yeast.Gene regulation and systems biology · 2011Article
- MIG1 Glucose Repression in Metabolic Processes ofAvicenna journal of medical biotechnologyArticle
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Authors and funding
2 authors.
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Abstract
backgroundTranscriptional regulation involves protein-DNA and protein-protein interactions. Protein-DNA interactions involve reactants that are present in low concentrations, leading to stochastic behavior. In addition, multiple regulatory mechanisms are typically involved in transcriptional regulation. In the GAL regulatory system of Saccharomyces cerevisiae, the inhibition of glucose is accomplished through two regulatory mechanisms: one through the transcriptional repressor Mig1p, and the other through regulating the amount of transcriptional activator Gal4p. However, the impact of stochasticity in gene expression and hierarchy in regulatory mechanisms on the phenotypic level is not clearly understood.
resultsWe address the question of quantifying the effect of stochasticity inherent in these regulatory mechanisms on the performance of various genes under the regulation of Mig1p and Gal4p using a dynamic stochastic model. The stochastic analysis reveals the importance of both the mechanisms of regulation for tight expression of genes in the GAL network. The mechanism involving Gal4p is the dominant mechanism, yielding low variability in the expression of GAL genes. The mechanism involving Mig1p is necessary to maintain the switch-like response of certain GAL genes. The number of binding sites for Mig1p and Gal4p further influences the expression of the genes, with extra binding sites lowering the variability of expression. Our experiments involving growth on various substrates show that the trends predicted in mean expression and its variability are transmitted to the phenotypic level.
conclusionThe mechanisms involved in the transcriptional regulation and their variability set up a hierarchy in the phenotypic response to growth on various substrates. Structural motifs, such as the number of binding sites and the mechanism of regulation, determine the level of stochasticity and eventually, the phenotypic response.
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