ArticleBMC plant biology2008
Assessment of allelic diversity in intron-containing Mal d 1 genes and their association to apple allergenicity.
Article in BMC plant biology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- Multianalytical Approach to Understand Polyphenol-Mal d 1 Interactions to Predict Their Impact on the Allergenic Potential of Apples.Journal of agricultural and food chemistry · 2024Article
- New Mass Spectrometric Approach to Quantify the Major Isoallergens of the Apple Allergen Mal d 1.Journal of agricultural and food chemistry · 2022Article
- Identification of allergenomic signatures in allergic and well-tolerated apple genotypes using LC-MS/MS.Food chemistry. Molecular sciences · 2022Article
- Microscale Thermophoresis Reveals Oxidized Glutathione as High-Affinity Ligand of Mal d 1.Foods (Basel, Switzerland) · 2021Article
- Apple allergy: Causes and factors influencing fruits allergenic properties-Review.Clinical and translational allergy · 2021Review
- Molecular and Immunological Identification of Low Allergenic Fruits among Old and New Apple Varieties.International journal of molecular sciences · 2021Article
- Tiered approach for the identification of Mal d 1 reduced, well tolerated apple genotypes.Scientific reports · 2020Article
- Allergen-specific immunotherapy with apples: selected cultivars could be a promising tool for birch pollen allergy.Journal of the European Academy of Dermatology and Venereology : JEADV · 2020Article
- Investigation of the Effects of Apple Trees Infection byReports of biochemistry & molecular biology · 2020Article
- A qRT-PCR assay for the expression of all Mal d 1 isoallergen genes.BMC plant biology · 2013Article
- Genomic organisation of the Mal d 1 gene cluster on linkage group 16 in apple.Molecular breeding : new strategies in plant improvement · 2012Article
- Scientific Opinion on the assessment of allergenicity of GM plants and microorganisms and derived food and feed.EFSA journal. European Food Safety Authority · 2010Article
- Characterization of PR-10 genes from eight Betula species and detection of Bet v 1 isoforms in birch pollen.BMC plant biology · 2009Article
- Genomic characterization of putative allergen genes in peach/almond and their synteny with apple.BMC genomics · 2008Article
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9 authors.
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Abstract
backgroundMal d 1 is a major apple allergen causing food allergic symptoms of the oral allergy syndrome (OAS) in birch-pollen sensitised patients. The Mal d 1 gene family is known to have at least 7 intron-containing and 11 intronless members that have been mapped in clusters on three linkage groups. In this study, the allelic diversity of the seven intron-containing Mal d 1 genes was assessed among a set of apple cultivars by sequencing or indirectly through pedigree genotyping. Protein variant constitutions were subsequently compared with Skin Prick Test (SPT) responses to study the association of deduced protein variants with allergenicity in a set of 14 cultivars.
resultsFrom the seven intron-containing Mal d 1 genes investigated, Mal d 1.01 and Mal d 1.02 were highly conserved, as nine out of ten cultivars coded for the same protein variant, while only one cultivar coded for a second variant. Mal d 1.04, Mal d 1.05 and Mal d 1.06 A, B and C were more variable, coding for three to six different protein variants. Comparison of Mal d 1 allelic composition between the high-allergenic cultivar Golden Delicious and the low-allergenic cultivars Santana and Priscilla, which are linked in pedigree, showed an association between the protein variants coded by the Mal d 1.04 and -1.06A genes (both located on linkage group 16) with allergenicity. This association was confirmed in 10 other cultivars. In addition, Mal d 1.06A allele dosage effects associated with the degree of allergenicity based on prick to prick testing. Conversely, no associations were observed for the protein variants coded by the Mal d 1.01 (on linkage group 13), -1.02, -1.06B, -1.06C genes (all on linkage group 16), nor by the Mal d 1.05 gene (on linkage group 6).
conclusionProtein variant compositions of Mal d 1.04 and -1.06A and, in case of Mal d 1.06A, allele doses are associated with the differences in allergenicity among fourteen apple cultivars. This information indicates the involvement of qualitative as well as quantitative factors in allergenicity and warrants further research in the relative importance of quantitative and qualitative aspects of Mal d 1 gene expression on allergenicity. Results from this study have implications for medical diagnostics, immunotherapy, clinical research and breeding schemes for new hypo-allergenic cultivars.
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