ArticleBiomaterials2009
Gene delivery in vitro and in vivo from bioreducible multilayered polyelectrolyte films of plasmid DNA.
Article in Biomaterials, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
24 citing papers in PubMed, 110 citations in OpenAlex.
- Article
- The hydroxyapatite modified 3D printed poly L-lactic acid porous screw in reconstruction of anterior cruciate ligament of rabbit knee joint: a histological and biomechanical study.BMC musculoskeletal disorders · 2023Article
- Polyelectrolyte Multilayers: An Overview on Fabrication, Properties, and Biomedical and Environmental Applications.Materials (Basel, Switzerland) · 2021Review
- Nanomedicine for the SARS-CoV-2: State-of-the-Art and Future Prospects.International journal of nanomedicine · 2021Review
- Fluorescence in "Nonfluorescent" Polymers.ACS omega · 2020Review
- Layer-by-layer DNA films incorporating highly transfecting bioreducible poly(amido amine) and polyethylenimine for sequential gene delivery.International journal of nanomedicine · 2018Article
- Triggerable Degradation of Polyurethanes for Tissue Engineering Applications.ACS applied materials & interfaces · 2015Article
- Bioreducible polycations in nucleic acid delivery: past, present, and future trends.Macromolecular bioscience · 2014Review
- Controlled release of an anti-cancer drug from DNA structured nano-films.Scientific reports · 2014Article
- Particles from preformed polymers as carriers for drug delivery.EXCLI journal · 2014Review
- Multiplex blood reporters for simultaneous monitoring of cellular processes.Analytical chemistry · 2013Article
- Effect of cell membrane thiols and reduction-triggered disassembly on transfection activity of bioreducible polyplexes.European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences · 2012Article
- Article
- A pH-sensitive multifunctional gene carrier assembled via layer-by-layer technique for efficient gene delivery.International journal of nanomedicine · 2012Article
- Small-interfering RNA-eluting surfaces as a novel concept for intravascular local gene silencing.Molecular medicine (Cambridge, Mass.) · 2011Article
- Delivery of plasmid DNA to vascular tissue in vivo using catheter balloons coated with polyelectrolyte multilayers.Biomaterials · 2011Article
- Enhanced gene and siRNA delivery by polycation-modified mesoporous silica nanoparticles loaded with chloroquine.Pharmaceutical research · 2010Article
- Tuning the mechanical properties of bioreducible multilayer films for improved cell adhesion and transfection activity.Biomaterials · 2010Article
- DNA release dynamics from bioreducible layer-by-layer films.Langmuir : the ACS journal of surfaces and colloids · 2010Article
- Cross-linked bioreducible layer-by-layer films for increased cell adhesion and transgene expression.The journal of physical chemistry. B · 2010Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Layer-by-layer (LbL) films were assembled on flexible stainless steel substrate using plasmid DNA and reducible hyperbranched poly(amido amine) (RHB) polycation. The films were characterized by XPS and their disassembly in reducing conditions confirmed by ellipsometry. Fibroblast and smooth muscle cell attachment and proliferation on DNA/RHB films were indistinguishable from those on control DNA/poly(ethylenimine) (PEI) films. In vitro transfection activity was evaluated using reporter plasmids encoding for secreted alkaline phosphatase (SEAP) and green fluorescent protein (GFP). DNA/RHB films showed higher and longer lasting transfection activity than control DNA/PEI films using SEAP plasmid. It was revealed through the use of GFP plasmid that DNA/RHB films transfected almost the entire cell population growing on the films. In vivo transfection activity was evaluated by subcutaneously implanting a stainless steel substrate coated with the DNA/RHB films containing SEAP plasmid DNA and measuring the levels of SEAP secreted into the blood circulation of rats. It was found that the plasma levels of SEAP peaked at approximately 160 ng SEAP/mL five days post-implantation.
Indexed as
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.