ArticleCytometry. Part A : the journal of the International Society for Analytical Cytology2009
Sorting and expansion of murine embryonic stem cell colonies using micropallet arrays.
Article in Cytometry. Part A : the journal of the International Society for Analytical Cytology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
15 citing papers in PubMed, 22 citations in OpenAlex.
- A technology of a different sort: microraft arrays.Lab on a chip · 2021Review
- Highly efficient cellular cloning using Ferro-core Micropallet Arrays.Scientific reports · 2017Article
- Optical painting and fluorescence activated sorting of single adherent cells labelled with photoswitchable Pdots.Nature communications · 2016Article
- Micropatterned photodegradable hydrogels for the sorting of microbeads and cells.Angewandte Chemie (International ed. in English) · 2013Article
- Efficient division and sampling of cell colonies using microcup arrays.The Analyst · 2013Article
- Impact of release dynamics of laser-irradiated polymer micropallets on the viability of selected adherent cells.Journal of the Royal Society, Interface · 2012Article
- Polystyrene-coated micropallets for culture and separation of primary muscle cells.Analytical and bioanalytical chemistry · 2012Article
- Patterning pallet arrays for cell selection based on high-resolution measurements of fluorescent biosensors.Analytica chimica acta · 2011Article
- Targeted messenger RNA profiling of transfected breast cancer gene in a living cell.Analytical biochemistry · 2011Article
- Selection and separation of viable cells based on a cell-lethal assay.Analytical chemistry · 2011Article
- Transparent magnetic photoresists for bioanalytical applications.Biomaterials · 2010Article
- Contact printing of arrayed microstructures.Analytical and bioanalytical chemistry · 2010Article
- Microcup arrays for the efficient isolation and cloning of cells.Analytical chemistry · 2010Article
- In situ roughening of polymeric microstructures.ACS applied materials & interfaces · 2010Article
- Enrichment and expansion of cells using antibody-coated micropallet arrays.Cytometry. Part A : the journal of the International Society for Analytical Cytology · 2009Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Isolation of cell colonies is an essential task in most stem cell studies. Conventional techniques for colony selection and isolation require significant time, labor, and consumption of expensive reagents. New microengineered technologies hold the promise for improving colony manipulation by reducing the required manpower and reagent consumption. Murine embryonic stem cells were cultured on arrays composed of releasable elements termed micropallets created from a biocompatible photoresist. Micropallets containing undifferentiated colonies were released using a laser-based technique followed by cell collection and expansion in culture. The micropallet arrays provided a biocompatible substrate for maintaining undifferentiated murine stem cells in culture. A surface coating of 0.025% gelatin was shown to be optimal for cell culture and collection. Arrays composed of surface-roughened micropallets provided further improvements in culture and isolation. Colonies of viable stem cells were efficiently isolated and collected. Colonies sorted in this manner were shown to remain undifferentiated even after collection and further expansion in culture. Qualitative and quantitative analyses of sorting, collection efficiency, and cell viability after release and expansion of stem cell colonies demonstrated that the micropallet array technology is a promising alternative to conventional sorting methods for stem cell applications.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.