ArticlePlant molecular biology2009
The A622 gene in Nicotiana glauca (tree tobacco): evidence for a functional role in pyridine alkaloid synthesis.
Article in Plant molecular biology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
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41 citing papers in PubMed, 110 citations in OpenAlex.
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- Auxin-Induced Nicotine Inhibition Is Mediated by NaARF5 Through the Suppression of NaERF1-Like Expression and Interaction With NaERF1-Like in Nicotiana attenuata.Plant biotechnology journal · 2026Article
- Forward genetic screening of mutants of the wild diploid ancestor Nicotiana sylvestris identifies aspartate oxidase 2 as a key gene for nicotine biosynthesis in cultivated allotetraploid tobacco (Nicotiana tabacum).The Plant journal : for cell and molecular biology · 2026Article
- Establishing a comprehensive web-based analysis platform for Nicotiana benthamiana genome and transcriptome.The Plant journal : for cell and molecular biology · 2025Article
- DNA methylation valley as a distinguishing feature occurs in root-specific expressed nicotine-related genes inFrontiers in plant science · 2025Article
- Development of an RNA virus vector for non-transgenic genome editing in tobacco and generation ofaBIOTECH · 2024Article
- Genetic regulation and manipulation of nicotine biosynthesis in tobacco: strategies to eliminate addictive alkaloids.Journal of experimental botany · 2024Review
- Proteomic and metabolomic insights into the impact of topping treatment on cigar tobacco.Frontiers in plant science · 2024Article
- Suppression of pyrrolidine ring biosynthesis and its effects on gene expression and subsequent accumulation of anatabine in leaves of tobacco (N. tabacum L.).BMC genomics · 2023Article
- Fractionation and Extraction Optimization of Potentially Valuable Compounds and Their Profiling in Six Varieties of TwoMolecules (Basel, Switzerland) · 2022Article
- Editing ofMolecular breeding : new strategies in plant improvement · 2022Article
- AJournal of advanced research · 2022Article
- NtMYB305a binds to the jasmonate-responsive GAG region of NtPMT1a promoter to regulate nicotine biosynthesis.Plant physiology · 2022Article
- Success of microbial genes based transgenic crops: Bt and beyond Bt.Molecular biology reports · 2021Review
- Effect of the over-dominant expression of proteins on nicotine heterosis via proteomic analysis.Scientific reports · 2021Article
- PseudomonasFrontiers in microbiology · 2021Article
- Establishment of in vitro culture system for3 Biotech · 2020Article
- Transcriptome analysis reveals key genes involved in the regulation of nicotine biosynthesis at early time points after topping in tobacco (Nicotiana tabacum L.).BMC plant biology · 2020Article
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Nicotiana glauca (Argentinean tree tobacco) is atypical within the genus Nicotiana, accumulating predominantly anabasine rather than nicotine and/or nornicotine as the main component of its leaf pyridine alkaloid fraction. The current study examines the role of the A622 gene from N. glauca (NgA622) in alkaloid production and utilises an RNAi approach to down-regulate gene expression and diminish levels of A622 protein in transgenic tissues. Results indicate that RNAi-mediated reduction in A622 transcript levels markedly reduces the capacity of N. glauca to produce anabasine resulting in plants with scarcely any pyridine alkaloids in leaf tissues, even after damage to apical tissues. In addition, analysis of hairy roots containing the NgA622-RNAi construct shows a substantial reduction in both anabasine and nicotine levels within these tissues, even if stimulated with methyl jasmonate, indicating a role for the A622 enzyme in the synthesis of both alkaloids in roots of N. glauca. Feeding of Nicotinic Acid (NA) to hairy roots of N. glauca containing the NgA622-RNAi construct did not restore capacity for synthesis of anabasine or nicotine. Moreover, treatment of these hairy root lines with NA did not lead to an increase in anatabine levels, unlike controls. Together, these results strongly suggest that A622 is an integral component of the final enzyme complex responsible for biosynthesis of all three pyridine alkaloids in Nicotiana.
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