ArticleApplied and environmental microbiology2009
The group III two-component histidine kinase of filamentous fungi is involved in the fungicidal activity of the bacterial polyketide ambruticin.
Article in Applied and environmental microbiology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 46 citations in OpenAlex.
- A General Strategy for the Synthesis of Jerangolids Enabled by π-allyl Stille Coupling.Chemistry (Weinheim an der Bergstrasse, Germany) · 2026Article
- Hybrid histidine kinases and antifungal warfare in thermal dimorphic fungi.Antimicrobial agents and chemotherapy · 2026Review
- Total Synthesis of Jerangolid B via spOrganic letters · 2025Article
- The FvHOG1 pathway is essential for stress responses, fungicide resistance, fumonisin B1 production and pathogenesis in Fusarium verticillioides.Crop health · 2025Article
- The Corey-Seebach Reagent in the 21st Century: A Review.Molecules (Basel, Switzerland) · 2023Review
- Distinct role of HAMP and HAMP-like linker domains in regulating the activity of Hik1p, a hybrid histidine kinase 3 from Magnaporthe oryzae.Molecular genetics and genomics : MGG · 2021Article
- Enantioselective Total Synthesis of the Putative Biosynthetic Intermediate Ambruticin J.Chemistry (Weinheim an der Bergstrasse, Germany) · 2021Article
- Non-Target Site Mechanisms of Fungicide Resistance in Crop Pathogens: A Review.Microorganisms · 2021Review
- The Journey from Two-Step to Multi-Step Phosphorelay Signaling Systems.Current genomics · 2021Review
- Responses to Hydric Stress in the Seed-Borne Necrotrophic FungusFrontiers in microbiology · 2019Article
- The Genomes of Three Uneven Siblings: Footprints of the Lifestyles of Three Trichoderma Species.Microbiology and molecular biology reviews : MMBR · 2016Review
- Phenylpyrroles: 30 Years, Two Molecules and (Nearly) No Resistance.Frontiers in microbiology · 2016Review
- Insights from the genome of Ophiocordyceps polyrhachis-furcata to pathogenicity and host specificity in insect fungi.BMC genomics · 2015Article
- Glucosinolate-derived isothiocyanates impact mitochondrial function in fungal cells and elicit an oxidative stress response necessary for growth recovery.Frontiers in plant science · 2015Article
- Histidine kinases mediate differentiation, stress response, and pathogenicity in Magnaporthe oryzae.MicrobiologyOpen · 2014Article
- Deletion of the HAMP domains from the histidine kinase CaNik1p of Candida albicans or treatment with fungicides activates the MAP kinase Hog1p in S. cerevisiae transformants.BMC microbiology · 2013Article
- Article
- Role of mannitol metabolism in the pathogenicity of the necrotrophic fungus Alternaria brassicicola.Frontiers in plant science · 2013Article
- Dehydrin-like proteins in the necrotrophic fungus Alternaria brassicicola have a role in plant pathogenesis and stress response.PloS one · 2013Article
- The Arabidopsis thaliana-Alternaria brassicicola pathosystem: A model interaction for investigating seed transmission of necrotrophic fungi.Plant methods · 2012Article
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Authors and funding
8 authors at 2 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have shown that the plant pathogen Alternaria brassicicola exhibited very high susceptibility to ambruticin VS4 and to a lesser extent to the phenylpyrrole fungicide fludioxonil. These compounds are both derived from natural bacterial metabolites with antifungal properties and are thought to exert their toxicity by interfering with osmoregulation in filamentous fungi. Disruption of the osmosensor group III histidine kinase gene AbNIK1 (for A. brassicola NIK1) resulted in high levels of resistance to ambruticin and fludioxonil, while a mutant isolate characterized by a single-amino-acid substitution in the HAMP domain of the kinase only exhibited moderate resistance. Moreover, the natural resistance of Saccharomyces cerevisiae to these antifungal molecules switched to sensitivity in strains expressing AbNIK1p. We also showed that exposure to fludioxonil and ambruticin resulted in abnormal phosphorylation of a Hog1-like mitogen-activated protein kinase (MAPK) in A. brassicicola. Parallel experiments carried out with wild-type and mutant isolates of Neurospora crassa revealed that, in this species, ambruticin susceptibility was dependent on the OS1-RRG1 branch of the phosphorelay pathway downstream of the OS2 MAPK cascade but independent of the yeast Skn7-like response regulator RRG2. These results show that the ability to synthesize a functional group III histidine kinase is a prerequisite for the expression of ambruticin and phenylpyrrole susceptibility in A. brassicicola and N. crassa and that, at least in the latter species, improper activation of the high-osmolarity glycerol-related pathway could explain their fungicidal properties.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.