ArticleInvestigative ophthalmology & visual science2009
Studies on bicarbonate transporters and carbonic anhydrase in porcine nonpigmented ciliary epithelium.
Article in Investigative ophthalmology & visual science, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 41 citations in OpenAlex.
- Article
- Carbonic Anhydrase Inhibitor Modulation of Intraocular Pressure Is Independent of Soluble Adenylyl Cyclase.Journal of ocular pharmacology and therapeutics : the official journal of the Association for Ocular Pharmacology and Therapeutics · 2023Article
- Mechanical Stretch Activates TRPV4 and Hemichannel Responses in the Nonpigmented Ciliary Epithelium.International journal of molecular sciences · 2023Article
- Ocular surface disease: a known yet overlooked side effect of topical glaucoma therapy.Frontiers in toxicology · 2023Review
- A Mathematical Model of Aqueous Humor Production and Composition.Investigative ophthalmology & visual science · 2022Article
- The therapeutic importance of acid-base balance.Biochemical pharmacology · 2021Review
- A Theoretical Approach for the Electrochemical Characterization of Ciliary Epithelium.Life (Basel, Switzerland) · 2020Article
- Trabecular Meshwork TREK-1 Channels Function as Polymodal Integrators of Pressure and pH.Investigative ophthalmology & visual science · 2019Article
- Overexpression of carbonic anhydrase 1 in pterygium.International journal of ophthalmology · 2016Article
- Discovery of novel inhibitors for the treatment of glaucoma.Expert opinion on drug discovery · 2015Review
- Genistein induces increase in fluid pH, Na+ and HCO3(-) concentration, SLC26A6 and SLC4A4 (NBCe1)-B expression in the uteri of ovariectomized rats.International journal of molecular sciences · 2014Article
- Nonpigmented ciliary epithelial cells respond to acetazolamide by a soluble adenylyl cyclase mechanism.Investigative ophthalmology & visual science · 2014Article
- DIDS inhibits Na-K-ATPase activity in porcine nonpigmented ciliary epithelial cells by a Src family kinase-dependent mechanism.American journal of physiology. Cell physiology · 2013Article
- The divergence, actions, roles, and relatives of sodium-coupled bicarbonate transporters.Physiological reviews · 2013Review
- Functional Roles of Electrogenic Sodium Bicarbonate Cotransporter NBCe1 in Ocular Tissues.The open ophthalmology journal · 2012Article
- Responses of sodium-hydrogen exchange to nitric oxide in porcine cultured nonpigmented ciliary epithelium.Investigative ophthalmology & visual science · 2009Article
- Elevated hydrostatic pressure activates sodium/hydrogen exchanger-1 in rat optic nerve head astrocytes.American journal of physiology. Cell physiology · 2009Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
purposeBicarbonate transport plays a role in aqueous humor (AH) secretion. The authors examined bicarbonate transport mechanisms and carbonic anhydrase (CA) in porcine nonpigmented ciliary epithelium (NPE).
methodsCytoplasmic pH (pH(i)) was measured in cultured porcine NPE loaded with BCECF. Anion exchanger (AE), sodium bicarbonate cotransporter (NBC), and CA were examined by RT-PCR and immunolocalization. AH secretion was measured in the intact porcine eye using a fluorescein dilution technique.
resultsAnion exchanger AE2, CAII, and CAIV were abundant in the NPE layer. In cultured NPE superfused with a CO(2)/HCO(3)(-)-free HEPES buffer, exposure to a CO(2)/HCO(3)(-)-containing buffer caused rapid acidification followed by a gradual increase in pH(i). Subsequent removal of CO(2)/HCO(3)(-) with HEPES buffer caused rapid alkalinization followed by a gradual decrease in pH(i). The rate of gradual alkalinization after the addition of HCO(3)(-)/CO(2) was inhibited by sodium-free conditions, DIDS, and the CA inhibitors acetazolamide and methazolamide but not by the Na-H exchange inhibitor dimethylamiloride or low-chloride buffer. The phase of gradual acidification after removal of HCO(3)(-)/CO(2) was inhibited by DIDS, acetazolamide, methazolamide, and low-chloride buffer. DIDS reduced baseline pH(i). In the intact eye, DIDS and acetazolamide reduced AH secretion by 25% and 44%, respectively.
conclusionsThe results suggest the NPE uses a Na(+)-HCO(3)(-) cotransporter to import bicarbonate and a Cl(-)/HCO(3)(-) exchanger to export bicarbonate. CA influences the rate of bicarbonate transport. AE2, CAII, and CAIV are enriched in the NPE layer of the ciliary body, and their coordinated function may contribute to AH secretion by effecting bicarbonate transport into the eye.
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