ArticlePlant physiology2009
Combined proteomic and cytological analysis of Ca2+-calmodulin regulation in Picea meyeri pollen tube growth.
Article in Plant physiology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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Who cites it
35 citing papers in PubMed, 63 citations in OpenAlex.
- Identification of circRNAs in industrial hemp (Frontiers in plant science · 2026Article
- Extracting and Quantifying Specific Pollen Proteins by Western Blot.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Calmodulin1-Calmodulin Binding Transcription Activator (CAM1-CAMTA) negatively regulate the transcription of Fluoride Export Gene 1 (FEX1) to mediate fluoride transport in tea (Camellia sinensis).Journal of experimental botany · 2025Article
- A regulatory network involving calmodulin controls phytosulfokine peptide processing during drought-induced flower abscission.The Plant cell · 2024Article
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- Distribution of exchangeable CaScientific reports · 2024Article
- A comprehensive overview of omics-based approaches to enhance biotic and abiotic stress tolerance in sweet potato.Horticulture research · 2024Article
- Transcriptomic analysis of tuberous root in two sweet potato varieties reveals the important genes and regulatory pathways in tuberous root development.BMC genomics · 2022Article
- Integrative transcriptome and proteome analyses provide deep insights into the molecular mechanism of salt tolerance in Limonium bicolor.Plant molecular biology · 2022Article
- Cotton proteomics: Dissecting the stress response mechanisms in cotton.Frontiers in plant science · 2022Review
- Pollen Germination and Pollen Tube Growth in Gymnosperms.Plants (Basel, Switzerland) · 2021Review
- Integrated mRNA and miRNA transcriptome analysis reveals a regulatory network for tuber expansion in Chinese yam (Dioscorea opposita).BMC genomics · 2020Article
- Bipolar pollen germination in blue spruce (Picea pungens).Protoplasma · 2019Article
- Genome-wide identification and expression analysis of calmodulin-like (CML) genes in Chinese cabbage (Brassica rapa L. ssp. pekinensis).BMC genomics · 2017Article
- Gene expression profile indicates involvement of NO in Camellia sinensis pollen tube growth at low temperature.BMC genomics · 2016Article
- Pollen proteomics: from stress physiology to developmental priming.Plant reproduction · 2016Review
- Anion Channel Inhibitor NPPB-Inhibited Fluoride Accumulation in Tea Plant (Camellia sinensis) Is Related to the Regulation of Ca²⁺, CaM and Depolarization of Plasma Membrane Potential.International journal of molecular sciences · 2016Article
- Combined Cytological and Transcriptomic Analysis Reveals a Nitric Oxide Signaling Pathway Involved in Cold-Inhibited Camellia sinensis Pollen Tube Growth.Frontiers in plant science · 2016Article
- Article
- Cytological and Proteomic Analyses of Osmunda cinnamomea Germinating Spores Reveal Characteristics of Fern Spore Germination and Rhizoid Tip Growth.Molecular & cellular proteomics : MCP · 2015Article
Corrections and comments
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Authors and funding
9 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Ca2+-calmodulin (Ca2+-CaM) is a critical molecule that mediates cellular functions by interacting with various metabolic and signaling pathways. However, the protein expression patterns and accompanying serial cytological responses in Ca2+-CaM signaling deficiency remain enigmatic. Here, we provide a global analysis of the cytological responses and significant alterations in protein expression profiles after trifluoperazine treatment in Picea meyeri, which abrogates Ca2+-CaM signaling. Ninety-three differentially displayed proteins were identified by comparative proteomics at different development stages and were assigned to different functional categories closely related to tip growth machinery. The inhibition of Ca2+-CaM signaling rapidly induced an increase in extracellular Ca2+ influx, resulting in dramatically increased cytosolic Ca2+ concentrations and ultrastructural abnormalities in organelles as the primary responses. Secondary and tertiary alterations included actin filament depolymerization, disrupted patterns of endocytosis and exocytosis, and cell wall remodeling, ultimately resulting in perturbed pollen tube extension. In parallel with these cytological events, time-course experiments revealed that most differentially expressed proteins showed time-dependent quantitative changes (i.e. some signaling proteins and proteins involved in organelle functions and energy production changed first, followed by alterations in proteins related to cytoskeletal organization, secretory pathways, and polysaccharide synthesis). Taken together, Ca2+-CaM dysfunction induced serial cytological responses and temporal changes in protein expression profiles, indicating the pivotal role of Ca2+-CaM in the regulation of tip growth machinery.
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